Abstract
Four diploid somatic recombinants were isolated from hybrids either between or within two diploid cell lines of a male Indian muntjac after HVJ virus-mediated cell fusion. Both parental lines had a normal male karyotype, 7,XY1Y2, in which the largest autosomal pair was heteromorphic with respect to the size of the secondary constriction (1h+/1h−), C bands, and nucleolar organizers. Of the four recombinants, three showed a 6,XX,1h+/1h+ or 1h+/1h− karyotype, the remaining one a 7,XY1Y2,1h+/1h+. No late-replicating X chromosome was found in the XX recombinants, although it was demonstrated in the natural XX line, suggesting the presence of two active X chromosomes in the former. The G6PD, PGK, and HPRT activities were proportional to the number of active X chromosomes present in all cell types examined, indicating their X-linkage, whereas the same level of activities obtained for LDH and 6PGD indicated their autosomal linkage.
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Yoshida, M.C., Sasaki, M. Euploid somatic recombinants with two active X or XY1Y2 chromosomes isolated from cultured male Indian muntjac cells after HVJ virus fusion, and their use for gene assignment. Somat Cell Mol Genet 4, 437–450 (1978). https://doi.org/10.1007/BF01538865
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DOI: https://doi.org/10.1007/BF01538865