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Isolation of chloramphenicol-resistant variants from a human cell line

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Somatic Cell Genetics

Abstract

Variant clones resistant to 40 μg/ml chloramphenicol were isolated from the human cell line VA 2-B after treatment with either ethyl methanesulfonate or N-methyl-N′-nitro-N-nitrosoguanidine. Among 17 clones analyzed, one variant, CAP-23, was investigated in detail.

CAP-23 cells in the presence of 40 or 100 μg/ml chloramphenicol grew at essentially the same rate as cells in the absence of the drug; chloramphenicol resistance persisted even after 20 generations in the absence of the drug. No obvious morphological changes in mitochondria were observed by electron microscopy of thin sections of CAP-23 cells. In vivo mitochondrial protein synthesis in CAP-23 cells was inhibited little, if any, by chloramphenicol, and the variant showed partial cross resistance to mikamycin and carbomycin. In vitro protein synthesis in mitochondria isolated from CAP-23 cells showed, likewise, low levels of inhibition by chloramphenicol. This suggests that the drug resistance of the variant CAP-23 is due to altered mitochondria.

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Authors and Affiliations

  1. Division of Biology, California Institute of Technology, Pasadena, California

    Carolyn H. Mitchell, James M. England & Giuseppe Attardi

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  1. Carolyn H. Mitchell
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  2. James M. England
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  3. Giuseppe Attardi
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Mitchell, C.H., England, J.M. & Attardi, G. Isolation of chloramphenicol-resistant variants from a human cell line. Somat Cell Mol Genet 1, 215–234 (1975). https://doi.org/10.1007/BF01538447

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  • DOI: https://doi.org/10.1007/BF01538447

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