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Evaluation of VP 2 epitopes of infectious bursal disease virus using in vitro expression and radioimmunoprecipitation

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Summary

A clone (pV 17-7) spanning a portion of the VP2 gene of infectious bursal disease virus (IBDV) was selected from a cDNA library produced using the variant A virus strain. This clone was expressed in vitro and the protein products were immunoprecipitated with various virus-neutralizing antisera made against 6 different strains of IBDV. The antisera made against 4 variant strains immunoprecipitated the translation products from the pV 17-7 clone, but the antisera to the classic STC virus and the serotype 2 OH virus did not immunoprecipitate the pV 17-7 translation products.

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Authors and Affiliations

  1. Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio

    J. M. Crisman, R. J. Jackwood & D. J. Jackwood

  2. National Cancer Institute Frederick Cancer Research Facility, Frederick, Maryland, USA

    D. P. Lana

Authors
  1. J. M. Crisman
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  2. R. J. Jackwood
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  3. D. P. Lana
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  4. D. J. Jackwood
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Crisman, J.M., Jackwood, R.J., Lana, D.P. et al. Evaluation of VP 2 epitopes of infectious bursal disease virus using in vitro expression and radioimmunoprecipitation. Archives of Virology 128, 333–344 (1993). https://doi.org/10.1007/BF01309443

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  • DOI: https://doi.org/10.1007/BF01309443

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