Abstract
An automated derivatization with dabsyl chloride in combination with a high-performance liquid Chromatographic analysis is described for the determination of biogenic amines in complex matrices. The sample clean-up procedure consists of an ultrafiltration step, resulting in average recoveries for cheese in the range of 88% to 100%. A linear relation between the area of the peak and amine concentration was observed between 0.5 and 500 pmol for all amines under investigation and the detection limits ranged between 0.34 and 0.76 pmol. The average repeatability of both the performance of the Chromatographic determination and the whole method, including sample preparation, examined using cheese samples containing different amounts of biogenic amines was found to be between 2.0% and 3.7%. The method was applied to the analysis of several types of food and feed, selected examples are given by the separation of dabsyl derivates from cheese, wine, and salami-type sausage.
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References
Askar A, Treptow H (1986) Biogene Amine in Lebensmitteln. Ulmer, Stuttgart
Guggenheim M (1951) Die biogenen Amine und ihre Bedeutung für die Physiologie und Pathologie des pflanzlichen und tierischen Stoffwechsels. Karger, Basel
Askar A (1982) Ernähr Umsch 29: 143–148
Rice SL, Eitenmiller RR, Koeler PE (1976) J Milk Food Technol 39: 166–169
Beutling D, Scheibner IJ, Scheibner G (1984) Mh Veterinärmed 39: 231–237
Edards ST, Sandine W (1981) J Dairy Sci 64: 2431–2438
Lüthy J, Schlatter Ch (1983) Z Lebensm Unters Forsch 177: 439–443
Seiler N, Demisch L, Schneider H (1971) Angew Chem Int Ed Eng 10: 51–66
Pfannhauser W, Pechanek U (1984) Z Gesamte Hyg Grenzgeb 30: 66–76
Askar A, Bielig HJ (1974) Alimenta 13: 3–9
Taylor SL, Keefe TJ, Windham ES, Howell JF (1982) J Food Protect 45: 455–457
Joosten HMLJ (1988) Neth Milk Dairy J 42: 25–42
ten Brink B, Damink C, Joosten HMLJ, Huis in't Veld JKJ (1990) Int J Food Microbiol 11: 73–84
Pechanek U, Woidich H, Pfannhauser W, Blaicher G (1980) Nutrion 4: 58–61
Koehler PE, Eitenmiller RR (1978) J Food Sci 43: 1245–1247
Binder E (1983) Milchwirtsch Berichte 75: 229–240
Antila P, Antila V, Mattila J, Hakkarainen H (1984) Milchwissenschaft 39: 81–84
Sieber R, Bilic N (1992) Schweiz Landwirtsch Forsch 32: 33–58
Moret S, Bortolomeazzi R, Lercker G (1992) J Chromatogr 591: 175–180
van Boekel MAJS, Arentsen-Stasse AP (1987) J Chromatogr 389: 267–272
Hurst WJ (1990) J Liq Chromatogr 13: 1–23
Treptow H, Askar A (1990) Ernährungsforschung 14: 9–11, 14–17
Mietz JL, Karmas E (1978) J Assoc Off Anal Chem 61: 139–145
Rosier J, van Peteghem C (1988) Z Lebensm Unters Forsch 186: 25–28
Etter R, Dietrich S, Battaglia R (1990) Mitt Lebensm Hyg 81: 106–119
Plessi M, Ferioli V, Gamberini G, Monzani A (1990) Atti Soc Nat Mat Modena 121: 1–9
Outinen K, Vuorela H, Hinkkanen R, Hiltunen R (1990) Acta Pharm Fenn 99: 99–108
Bütikofer U, Fuchs D, Hurni D, Bosset OJ (1990) Mitt Lebensm Hyg 81: 120–133
Henry Chin KD, Koehler PE (1983) J Food Sci 48: 1826–1828
Hui JY, Taylor StL (1983) J Assoc Off Anal Chem 66: 853–857
Schwedt G, Bussemas HH (1977) Z Anal Chem 283: 23–28
Lin JK, Lai CC (1982) J Chromatogr 227B: 369–378
Fröhlich D, Battaglia R (1980) Mitt Lebensm Hyg 71: 38–44
Lehtonen P, Saarinen M, Vesanto M, Riekkola ML (1992) Z Lebensm Unters Forsch 194: 434–437
Seiler N (1986) J Chromatogr 379: 157–176
Chang JY, Knecht R, Braun D (1983) Methods Enzymol 91: 41–48
Nieder M, Hughes GJ, Frutiger S, Grom E (1990) Labor Praxis 14: 26–31
Schneider HJ (1989) Chromatographia 28: 45–48
Chang JY, Knecht R, Braun D (1981) Biochem J 199: 547–555
Chang JY, Martin P, Bernasconi R, Braun D (1981) FEBS Lett 132: 117–120
Krause I, Bockhardt A, Neckermann N, Henle T, Klostermeyer H (1995) J Chromatogr 715: 67–79
DIN 32 645. Nachweis-, Erfassungs- und Bestimmungsgrenze. Ermittlung unter Wiederholbedingungen. Begriffe, Verfahren, Auswertung (05/1994)
Bütikofer U (1995) First collaborative study on the determination of biogenic amines in standard solution, wine cheese and feed. FAM Federal Dairy Research Institute Report, CH-Liebfeld
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Bockhardt, A., Krause, I. & Klostermeyer, H. Determination of biogenic amines by RP-HPLC of the dabsyl derivates. Z Lebensm Unters Forch 203, 65–70 (1996). https://doi.org/10.1007/BF01267772
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DOI: https://doi.org/10.1007/BF01267772