Skip to main content
SpringerLink
Log in

Evaluation of silica cartridge purification and hemiacetal formation for liquid chromatographic determination of aflatoxins in corn

  • Published:
Archives of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

Rapid silica cartridge cleanup, acid-catalyzed conversion of aflatoxins B1 and G1 to hemiacetals, and reverse-phase liquid chromatography with fluorescence detection were evaluated for effectiveness in determining aflatoxins B1, B2, G1, and G2 in corn at concentrations ranging from 2 ng/g to 100 (μg/g. In testing the method, aflatoxins applied to silica cartridges were recovered at greater than 97.1% overall. Conversion of aflatoxins B1 and G1 to their hemiacetals was shown to be complete (150 μg of toxin per extract). Application of these techniques to spiked corn extracts yielded data indicating excellent repeatability of derivatization relative to paired standards; coefficients of variation ranged from 1.08% to 5.81%. The repeatability of the method with naturally contaminated corn was also excellent; coefficients of variation ranged from 1.15% to 3.97%. Liquid Chromatographic determination of aflatoxins in corn using fluorescence detection was sensitive, accurate, and precise resulting in applicability from <1 ng/g of aflatoxin B1 to > 100,000 ng/g.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Beebe RM (1978) Reverse phase high pressure liquid chromatographic determination of aflatoxins in foods. J Assoc Offic Anal Chem 61:1347–1352

    Google Scholar 

  • Campbell AD, Francis OJ Jr, Beebe RA, Stoloff L (1984) Determination of aflatoxins in peanut butter using two liquid chromatographic methods. J Assoc Offic Anal Chem 67:312–316

    Google Scholar 

  • Chang HHL, DeVries JW, Hobbs WE (1979) Comparative study of two methods for extraction of aflatoxin from peanut meal and peanut butter. J Assoc Offic Anal Chem 62:1281–1284

    Google Scholar 

  • Cohen H, Lapointe M (1981) High pressure liquid chromatographic determination and fluorescence detection of aflatoxins in corn and dairy feeds. J Assoc Offic Anal Chem 64:1372–1376

    Google Scholar 

  • DeVries JW, Chang HL (1982) Comparison of rapid high pressure liquid chromatographic and CB methods for determination of aflatoxins in corn and peanuts. J Assoc Offic Anal Chem 65:206–209

    Google Scholar 

  • Diebold GJ, Karny N, Zare RN, Sietz LM (1979) Laser fluorometric determination of aflatoxin B1 in corn. J Assoc Offic Anal Chem 62:564–569

    Google Scholar 

  • Haghighi B, Thorpe C, Pohland AE, Barnett R (1981) Development of a sensitive high performance liquid chromatographic method for detection of aflatoxins in pistachio nuts. J Chromatogr 206:101–108

    PubMed  Google Scholar 

  • Hutchins JE, Hagler WM Jr (1983) Rapid liquid chromatographic determination of aflatoxins in heavily contaminated corn. J Assoc Offic Anal Chem 66:1458–1465

    Google Scholar 

  • Orti DL, Hill RH Jr, Liddle JA, Needham LL (1986) High performance liquid chromatography of mycotoxin metabolites in human urine. J Anal Toxicol 10:41–45

    PubMed  Google Scholar 

  • Roberts BA, Glancy EM, Patterson DSP (1981) Rapid economic method for determination of aflatoxin and ochratoxin in animal feedstuffs. J Assoc Offic Anal Chem 64:961–963

    Google Scholar 

  • Takahashi DM (1977) High pressure liquid chromatographic determination of aflatoxins in wines and other liquid products. J Assoc Offic Anal Chem 60:799–804

    Google Scholar 

  • Thean JE, Lorenz DR, Wilson DM, Rodgers K, Gueldner RC (1980) Extraction, cleanup, and quantitative determination of aflatoxins in corn. J Assoc Offic Anal Chem 63:631–633

    Google Scholar 

  • Wei RD, Chang SC, Lee SS (1980) High pressure liquid chromatographic determination of aflatoxins in soy sauce and fermented soybean paste. J Assoc Offic Anal Chem 63:1269–1274

    Google Scholar 

  • Williams S (1984) Official Methods of Analysis. 14th ed, ch 26, Assoc Offic Anal Chem, Arlington, VA

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Immunodiagnostics Department, Becton-Dickinson Research Center, 27709, Research Triangle Park, North Carolina, USA

    James E. Hutchins

  2. Mycotoxin Laboratory, Department of Poultry Science, North Carolina State University, Box 7636, 27695-7636, Raleigh, North Carolina, USA

    Y. Jennifer Lee & Winston M. Hagler Jr.

  3. School of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, 27606, Raleigh, North Carolina, USA

    Krystyna Tyczkowska

Authors
  1. James E. Hutchins
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Jennifer Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Krystyna Tyczkowska
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Winston M. Hagler Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Paper No. 10499 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, NC 27695-7601.

The use of trade names in this publication does not imply endorsement by the North Carolina Agricultural Research Service, nor criticism of similar ones not mentioned.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hutchins, J.E., Lee, Y.J., Tyczkowska, K. et al. Evaluation of silica cartridge purification and hemiacetal formation for liquid chromatographic determination of aflatoxins in corn. Arch. Environ. Contam. Toxicol. 18, 319–326 (1989). https://doi.org/10.1007/BF01062355

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01062355

Keywords

Search

Navigation