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Characteristics of a novel high viscosity polysaccharide, methylan, produced byMethylobacterium organophilum

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Summary

A new anionic high molecular weight polysaccharide, Methylan, was produced byMethylobacterium organophilum from methanol as a sole source of carbon and energy under the specific culture conditions. By GPC and light scattering, the molecular weight was determined to be 2–4×106 dalton and the distribution of molecular weight was very homogeneous. Methylan was composed of carbohydrate (80%), uronic acid (12%), protein (6%) and pyruvic acid (5%). The sugar composition of Methylan was identified as glucose, galactose and mannose with the approximate molar ratio of 2∶3∶2. Methylan solution showed a pseudoplastic non-Newtonian fluid property at the concentration above 0.05%. At the concentration of 1% Methylan solution, the consistency index was 18,000 centipoise which was almost 10 times higher than that of Xanthan and the flow behavior index was 0.15.

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Authors and Affiliations

  1. Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Cheongryang, P.O. Box 150, 130-012, Seoul, Korea

    Jung H. Kim

  2. Division de Procédes Biotechnologiques, Université de Technologie de Compiègne, B.P. 649, 60206, Compiègne Cédex, France

    J. M. Lebeault

Authors
  1. Joon H. Choi
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  2. Deok K. Oh
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  3. K. Oh
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  4. Jung H. Kim
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  5. J. M. Lebeault
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Choi, J.H., Oh, D.K., Oh, K. et al. Characteristics of a novel high viscosity polysaccharide, methylan, produced byMethylobacterium organophilum . Biotechnol Lett 13, 417–420 (1991). https://doi.org/10.1007/BF01030993

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