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The protective effect of antioxidants to a phototoxin-sensitive insect herbivore,Manduca sexta

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Abstract

Photo-activated plant secondary compounds have been shown to be toxic to many organisms including insects. Insect defenses include behavioral mechanisms such as light avoidance, as well as specific biochemical defenses such as antioxidants and antioxidant enzymes. These antioxidant defenses eliminate or quench the deleterious singlet oxygen and free radicals formed by these phototoxins. In this paper we examined the role of dietary antioxidants in protecting the phototoxin-sensitive insect herbivoreManduca sexta. Elevated dietary levels of the lipid-soluble antioxidantsΒ-carotene and vitamin E resulted in a concentration-dependent reduction in the mortality associated with treatment ofM. sexta larvae with the phototoxic thiopheneα-terthienyl. Elevated levels of dietary ascorbic acid had no effect, whereas reduced levels greatly increased the toxicity ofα-terthienyl. Tissue levels of antioxidants were shown to increase substantially in larvae fed antioxidant-supplemented diets. The results suggest that the ability to absorb and utilize plant-derived antioxidants could be an important defense against photo-activated plant secondary compounds and may have allowed some insects to exploit phototoxic plants.

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Author notes

  1. P. Fields

    Present address: Agriculture Canada Research Station, 195 Dafoe Rd., R3T 2M9, Winnipeg, Manitoba, Canada

Authors and Affiliations

  1. Ottawa-Carleton Institute of Biology, University of Ottawa, 30 George Glinski St., KIN 6N5, Ottawa, Ontario, Canada

    R. R. Aucoin, P. Fields, M. A. Lewis, B. J. R. Philogène & J. T. Arnason

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  1. R. R. Aucoin
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Aucoin, R.R., Fields, P., Lewis, M.A. et al. The protective effect of antioxidants to a phototoxin-sensitive insect herbivore,Manduca sexta . J Chem Ecol 16, 2913–2924 (1990). https://doi.org/10.1007/BF00979483

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