Abstract
A decrease in amino acid influx and exit in incubated slices when the temperature was lowered from 37 to 20°C was observed with all 16 amino acids examined at two concentrations (1 mM and 10 μM). The temperature dependence of cellular amino acid influx observed in slices in vitro contrasts with the absence of temperature dependence of capillary amino acid influx in the brain in vivo that we recently reported. The temperature effects in slices varied some-what among the various amino acid transport classes. With some amino acids (Phe, Val, Tyr, Asp, Glu), especially at lower concentrations, the greater inhibition of exit than of uptake resulted in an actual increase in tissue amino acid uptake at lower temperatures in long-term experiments. The results indicate heterogeneity in the effect of temperature on the various transport classes and show a difference between capillary and cellular amino acid transport in the brain.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Lajtha, A., andSershen, H. 1975. Changes in the rates of protein synthesis in the brain of goldfish at various temperatures. Life Sci. 17:1861–1868.
Emirbekov, E. Z., Sershen, H., andLajtha, A. 1977. Lack of effects of hypothermia on cerebral amino acid uptakein vivo. Brain Res. 125:187–191.
Guroff, G., King, W., andUdenfriend, S. 1961. The uptake of tyrosine by rat brainin vitro. J. Biol. Chem. 236:1773–1777.
Neame, K. D. 1961. Uptake of amino acids by mouse brain slices. J. Neurochem. 6:358–366.
Cohen, S. R. 1975. A comparison of the rate equations, kinetic parameters, and activation energies for the initial uptake ofl-lysine,l-valine, γ-aminobutyric acid, and α-aminoisobutyric acid by mouse brain slices. J. Membr. Biol. 22:53–72.
Banay-Schwartz, M., Teller, D. N., Gergely, A., andLajtha, A. 1974. The effects of metabolic inhibitors on amino acid uptake and the levels of ATP, Na+ and K+ in incubated slices of mouse brain. Brain Res. 71:117–131.
Sadasivudu, B., andLajtha, A. 1970. Metabolism of amino acids in incubated slices of mouse brain. J. Neurochem. 17:1299–1311.
Neidle, A., Kandera, J., andLajtha, A. 1975. Compartmentation and exchangeability of brain amino acids: Evidence from studies of transport into tissue slices. Arch. Biochem. Biophys. 169:397–405.
Levi, G., Blasberg, R., andLajtha, A. 1966. Substrate specificity of cerebral amino acids exitin vitro. Arch. Biochem. Biophys. 114:339–351.
Cohen, S. R., Stampleman, P. F., andLajtha, A. 1970. The temperature-dependent compartmentation of the “extracellular space” in mouse brain slices as revealed by the markers: inulin, sucrose,d-mannitol,d-sorbitol and sulfate. Brain Res. 21:419–434.
Blasberg, R. andLajtha, A. 1966. Heterogeneity of the mediated transport system of amino acid uptake in brain. Brain Res. 1:86–104.
Christensen, H. N. 1975. Biological Transport, Benjamin, Reading, Massachusetts.
Aschoff, J., andVon Saint Paul, U. 1976. Brain temperature in the unanaesthetized chicken: Its circadian rhythm of responsiveness to light. Brain Res. 101:1–9.
Cohen, S. R. 1973. The rate of respiration of mouse brain tissue in physiological and nonphysiological media as a function of temperature. Comp. Biochem. Physiol. 45A:1031–1045.
Jacquez, J. A., Sherman, J. H., andTerris, J. 1970. Temperature dependence of amino acid transport in Ehrlich ascites cells: With results which bear on the A-L distinction. Biochim. Biophys. Acta 203:150–166.
Navon, S., andLajtha, A. 1969. The uptake of amino acids by particulate fractions from brain. Biochim. Biophys. Acta 173:518–531.
Peterson, N. A., andRaghupathy, E., 1974. Postnatal changes in the temperature dependence of synaptosomal amino acid uptake. FEBS Lett. 48:176–178.
Oldendorf, W. H. 1971. Brain uptake of radio-labeled amino acids, amines, and hexoses after arterial injection. Am. J. Physiol. 221:1629–1639.
Sershen, H., andLajtha, A. 1976. Capillary transport of amino acids in the developing brain. Exp. Neurol. 53:465–474.
Lajtha, A., andSershen, H. 1975. Inhibition of amino acid uptake by the absence of Na+ in slices of brain. J. Neurochem. 24:667–672.
Wade, L. A., andKatzman, R. 1975. Synthetic amino acids and the nature ofl-DOPA transport at the blood-brain barrier. J. Neurochem. 25:837–842.
Oldendorf, W. H., andSzabo, J. 1976. Amino acid assignment to one of three blood-brain barrier amino acid carriers. Am. J. Physiol. 230:94–98.
Zanchin, G., Sershen, H., andLajtha, A. 1976. The effect of hyperosmolal urea on the transport of amino acids into rat brain. Exp. Neurol. 51:292–303.
Sershen, H., andLajtha, A. 1977. Low-affinity neutral amino acid transport systems in brain slices. Trans. Am. Soc. Neurochem. 8:207.
Go, K.-G., andPratt, J. J. 1975. The dependence of the blood to brain passage of radioactive sodium on blood pressure and temperature. Brain Res. 93:329–336.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Banay-Schwartz, M., Lajtha, K., Sershen, H. et al. Temperature dependence of amino acid transport in brain slices. Neurochem Res 2, 695–706 (1977). https://doi.org/10.1007/BF00963782
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00963782