Abstract
Plants ofLycopersicon esculentum (grown in greenhouses) andAnona cherimolia cultivated in southeastern Spain were examined for the presence of trypanosomatid flagellates. Kinetoplastid protozoa were found in the fruits but not in the phloem or other plant tissues. Parasites were detected from the onset of fruiting. Isolates were adapted to in vitro culturing in monophase media. The form and the structural organization was studied by scanning and transmission electron microscopy. The parasites showed an ultrastructural pattern similar to that of other species of the genusPhytomonas. In tomatoes experimentally inoculated with flagellates cultivated in vitro, we observed that the parasites did not lose their infectious capacity. Three strains of trypanosomatids of the genusPhytomonas, isolated from different species ofEuphorbia (E. characias andE. hyssopifolia) and fromCocos nucifera, were compared with our isolates by lectinagglutination tests. Our isolates were different from the two strains isolated fromEuphorbia, but with this technique we could not differentiate our isolates from those of the coconut, nor could we differentiate between the isolates, their ultrastructural similarity together with their similar behavior in the lectin-agglutination test suggesting that these isolates have a common origin.
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Sanchez-Moreno, M., Fernandez-Becerra, C., Mascaro, C. et al. Isolation, in vitro culture, ultrastructure study, and characterization by lectin-agglutination tests of Phytomonas isolated from tomatoes (Lycopersicon esculentum) and cherimoyas (Anona cherimolia) in southeastern Spain. Parasitol Res 81, 575–581 (1995). https://doi.org/10.1007/BF00932024
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DOI: https://doi.org/10.1007/BF00932024