Abstract
The Rcho-1 cell line, originally established from a rat choriocarcinoma, shows differentiation into placental trophoblastic giant cell-like cells and has been used to study the mechanism of placental function control. In the present study, we analysed the ganglioside composition of Rcho-1 cells by HPTLC orcinol/H2SO4, TLC/immunostaining and immunohistochemistry. Rcho-1 cells expressed GM3 and GD3 as the major gangliosides and CTH as major neutral glycolipid when they were cultured in growth medium (20% FCS) or transplanted beneath the kidney capsule. The expression of these gangliosides was strong in the undifferentiated small cells, whereas the completely differentiated giant cells showed poor staining with antibodies against the gangliosides. Under culture conditions to induce cell differentiation using horse serum (1–20% HS), the expression of GD3 was suppressed and re-expressed when the medium was changed to growth medium, suggesting that a change of ganglioside components may trigger and define the direction of terminal differentiation. Thus the composition of glycolipids is conserved in Rcho-1 cells and is similar to that of the rat placenta, where GM3 is dominant in mid-pregnancy and decreased in late pregnancy, whereas GD3 is low in mid-pregnancy and increased in late pregnancy.
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Itonori S, Shirai T, Kiso Y, Ohashi Y, Shiota K, Ogawa T (1995)Biochem J 307:399–405.
Soares MJ, Faria TN, Roby KF, Deb S (1991)Endocrine Rev 12:402–23.
Shiota K, Hirosawa M, Hattori N, Itonori S, Miura R, Noda K, Takahashi M, Ogawa T (1994)Endocrine J 41 (Suppl): S43-S56.
Svennerholm L (1965)Acta Chem Scand 19: 1506–7.
Taki T, Matsuo K, Yamamoto K, Matsubara T, Hayashi A, Abe T, Matsumoto M (1988)Lipids 23: 192–98.
Cross JC, Werb Z, Fisher SJ (1994)Science 266: 1508–18.
Mitchell MD, Trautman MS, Dudley DJ (1993)Placenta 14: 249–75.
Soares MJ, Faria TN, Hamlin GP, Lu X-J, Deb S (1993) InTrophoblast Cell Differentiation: Expression of the Placental Prolactin Family (Soares MJ, Handwerger S, Talamantes F eds) pp. 45–67. New York: Springer-Verlag.
Hakomori S-I (1993)Biochem Society Transactions 21: 583–95.
Hakomori S-I, Igarashi Y (1993)Advances Lipid Res 25: 147–62.
Hirosawa M, Miura R, Min K-S, Hattori N, Shiota K, Ogawa T (1994)Endocrine J 41: 387–97.
Hattori N, Wakimasu M, Takahashi M, Hirosawa M, Shiota K, Ogawa T (1993)Endocrine J 40: 727–35.
Furuyama N, Shiota K, Takahashi M (1991)Endocrinol Japan 38: 533–40.
Shiota K, Furuyama N, Takahashi M (1991)Endocrinol Japan 38: 541–49.
Faria TN, Deb S, Kwok SCM, Vandeputte M, Talamantes F, Soares MJ (1990)Endocrinology 127: 3131–37.
Teshima S, Shimosato Y, Koide T, Kuroki M, Kiruchi M, Aizawa M (1983)Jpn J Cancer Res: Gann 74: 205–12.
Hamlin GP, Lu XJ, Roby KF, Soares MJ (1994)Endocrinology 134: 2390–96.
Faria TN, Soares MJ (1991)Endocrinology 129: 2895–906.
Houghton AN, Mintzer D, Cordon-Cardo C, Welt S, Fliegel B, Vadhan S, Carswell E, Melamed MR, Oettgen HF, Old LJ (1985)Proc Natl Acad Sci USA 82: 1242–46.
Pukel CS, Lloyd KO, Travassos LR, Dippold WG, Oettgen HF, Old LJ (1982)J Exp Med 155: 1133–47.
Tai T, Kawashima I, Furukawa K, Lloyd KO (1988)Arch Biochem Biophys 260: 51–55.
Hidari K, Itonori S, Sanai Y, Ohashi M, Kasama T, Nagai Y (1991)J Biochem 110: 412–16.
Itonori S, Hidari K, Sanai Y, Taniguchi M, Nagai Y (1989)Glycoconj J 6: 551–60.
Magnani JL, Smith DF, Ginsburg V (1980)Anal Biochem 109: 399–402.
Higashi H, Fukui Y, Ueda S, Kato S, Hirabayashi Y, Matsumoto M, Naiki M (1984)J Biochem 95: 1517–20.
Kotani M, Kawashima I, Ozawa H, Terashima T, Tai T (1993)Glycobiology 3: 137–46.
Hansson GC, Wazniowska K, Rock JA, Ness PM, Kickler TS, Shirey RS, Niebyl JR, Zopf D (1988)Arch Biochem Biophys 260: 168–76.
Lampio A, Airaksinen A, Maaheimo H (1993)Glycoconj J 10: 165–69.
Rueda R, Tabsh K, Ladisch S (1993)FEBS Lett 328: 13–16.
Svejcar J, Ehrlich-Rogozinski S, Riedel D, Müthing J, Sharon N (1993)Glycoconj J 10: 247–50.
Hakomori S-I (1989)Adv Cancer Res 52: 257–331.
Portoukalian J, Zwingelstein G, Doré J-F (1979)Eur J Biochem 94: 19–23.
Furukawa K, Arita Y, Satomi N, Eisinger M, Lloyd KO (1990)Archiv Biochem Biophys 281: 70–75.
Hakomori S-I (1994)Prog Brain Res 101: 241–50.
Bremer EG, Schlessinger J, Hakomori S-I (1986)J Biol Chem 261: 2434–40.
Kanda T, Ariga T, Yamawaki M, Yu RK (1993)J Neurochem 61: 1969–72.
Hattori M, Horiuchi R (1992)Biochim Biophys Acta 1137: 101–6.
Hattori M, Kanzaki M, Kojima I, Horiuchi R (1994)Biochim Biophys Acta 1221: 47–53.
Norihisa Y, McVicar DW, Ghosh P, Houghton AN, Longo DL, Creekmore SP, Blake T, Ortaldo JR, Young HA (1994)J Immunol 152: 485–95.
Kopitz J, Reitzenstein C, Mühl C, Cantz M (1994)Biochem Biophys Res Commun 199: 1188–93.
Nakamura M, Tsunoda A, Sakoe K, Gu J, Nishikawa A, Taniguchi N, Saito M (1992)J Biol Chem 267: 23507–14.
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Shirai, T., Itonori, S., Tai, T. et al. Ganglioside composition of the rat choriocarcinoma cell line, Rcho-1. Glycoconjugate J 13, 415–421 (1996). https://doi.org/10.1007/BF00731474
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DOI: https://doi.org/10.1007/BF00731474