Abstract
A lectin preparation obtained fromTetracarpidium conophorum (Nigerian walnut) by affinity chromatography of seed extracts on lactose-agarose has been shown to contain two components by gel filtration on Sephadex G150. The larger componentTetracarpidium conophorum agglutinin I (TCAI) is a disulphide-bonded 70 kDa homodimer whereas the second component TCAII is a 34 kDa monomeric protein. Amino terminal aminoacid sequencing shows identity in TCAI and TCAII for the first fifteen residues after which the sequences diverge. TheN-terminal sequences of TCAI and TCAII show identity with sequences in the B-chains of ricin andRicinus communis agglutinin I (RCAI) in eleven of the initial fifteen residues. Thereafter TCAI appears to be homologous to the ricin B chain whereas TCAII is more homologous with the B chain of RCAI. A limited screening of the carbohydrate-binding specificity of TCAII by affinity chromatography of defined oligosaccharides on TCAII Sepharose columns shows that the binding specificity reported earlier for affinity purifiedTetracarpidium conophorum isolectins (Sato S, Animashaun T, Hughes RC (1991)J Biol Chem 266:11485–94) reflects the binding properties of TCAII which is the major isolectin in unfractionated lectin preparations.
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Togun RA, Animashaun T, Benzie CR, Kay JE (1988) InLectins: Biology-Biochemistry, Clinical Biochemistry. (Bøg-Hansen TC, Freed DLJ, eds). Vol. 6, pp. 369–72. Sigma Chemical Co.
Sato S, Animashaun T, Hughes RC (1991)J Biol Chem 266:11485–94.
Funatsu G, Yoshitake S, Funatsu M (1978)Agric Biol Chem 42:501–3.
Funatsu G, Kimura M, Funatsu M (1979)Agric Biol Chem 43:2221–4.
Bull H, Li S, Fowler E, Lin T (1980)Int J Pept Protein Res 16:208–18.
Roberts LM, Lamb FI, Pappin DJC, Lord JM (1985)J Biol Chem 260:15682–6.
Araki T, Funatsu G (1987)Biochim Biophys Acta 911:191–200.
Animashaun T, Hughes RC (1989)J Biol Chem 264:4657–63.
Aitken A, Geisow MJ, Findlay JBC, Holmes C, Yarwood A (1989) InProtein Sequencing: A Practical Approach (Geisow MJ, Findlay JBC, eds). pp. 46–68. Oxford: IRL Press.
Animashaun T (1983)Lectins, Vol. 3 (Bøg-Hansen TC, Spengler GA, eds). pp. 639–44. Berlin: Walter de Gruyter & Co.
Araki T, Funatsu G (1985)FEBS Lett 191:121–4.
Araki T, Yoshioka Y, Funatsu G (1986)Biochim Biophys Acta 872:277–85.
Takasaki S, Kobata A (1986)Biochemistry 25:5706–15.
Yamamoto K, Tsuji T, Itimura T, Osawa T (1981)Biochem J 195:701–13.
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Animashaun, T., Togun, R.A. & Hughes, R.C. Characterization of isolectins inTetracarpidium conophorum seeds (Nigerian walnut). Glycoconjugate J 11, 299–303 (1994). https://doi.org/10.1007/BF00731202
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DOI: https://doi.org/10.1007/BF00731202