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Characterization ofH-2 congenic strains using DNA markers

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Abstract

Congenic mouse strains are widely used in mapping traits to specific loci or short chromosomal regions. The precision of the mapping depends on the information available about the length of the differential segment—the segment introduced from the donor into the background strain. Until recently, very few markers flanking the differential locus were known and consequently the length of the foreign segment could only be determined imprecisely. Now, in an attempt to construct a map of the mouse chromosome 17, we have produced a set of DNA markers distributed along the chromosome. These markers provide a new opportunity to measure the length of the differential segment of the congenic strains and thus increase their usefulness for gene mapping. Here we examined the DNA of 96H-2 congenic strains using 30 DNA markers; of these, the most proximal is located roughly 1.5 centiMorgans (cM) from the centromere and the most distal is about 20 cM telomeric from theH-2 complex (the complex itself being some 20 cM from the centromere). The mapping depends on polymorphism among the input strains and can therefore establish only the minimal length of the differential segment. This point is emphasized by the fact that the average observed length of the differential segment is only about one half of the expected values.

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References

  • Duncan, W. R. and Klein, J.: Histocompatibility-2 system in wild mice. IX. Serological analysis of 13 new B10.W congenic lines.Immunogenetics 10: 45–65, 1980

    Google Scholar 

  • Figueroa, F., Golubić, M., Nižzetić, D., and Klein, J.: Evolution of mouse major histocompatibility complex genes borne byt chromosomes.Proc Natl Acad Sci USA 82: 2819–2823, 1985

    Google Scholar 

  • Figueroa, F., Kasahara, M., Tichy, H., Neufeld, E., Ritte, U., and Klein, J.: Polymorphism of unique noncoding DNA sequence in wild and laboratory mice.Genetics 117: 101–108, 1987

    Google Scholar 

  • Green, E. L. and Doolittle, D. P.: Systems of mating used in mammalian genetics.In W. J. Burdette (ed.):Methodology in Mammalian Genetics, pp. 3–55, Holden-Day, Inc., San Francisco, 1963

    Google Scholar 

  • Haldane, J. B. S.: Methods for the study of histocompatibility genes. Appendix.J Genetics 49: 104–108, 1948

    Google Scholar 

  • Klein, J.: Histocompatibility-2 system in wild mice. I. Identification of five new H-2 chromosomes.Transplantation 13: 291–299, 1972

    Google Scholar 

  • Klein, J.:Natural History of the Major Histocompatibility Complex. Wiley-Interscience, New York, 1986

    Google Scholar 

  • Klein, J.: Congenic and segregating inbred strains. Immunologically important loci.In M. F. Lyon and A. G. Searle (eds.):Genetic Variants and Strains of the Laboratory Mouse, 2nd edn, pp. 797–825, Oxford University Press, Oxford, 1989

    Google Scholar 

  • Klein, J. and Klein, D.: Mouse inbred and congenic strains.Methods Enzymol 150: 163–196, 1987

    Google Scholar 

  • Klein, D., Tewarson, S., Figueroa, F., and Klein, J.: The minimal length of the differential segment inH-2 congenic lines.Immunogenetics 16: 319–328, 1982a

    Google Scholar 

  • Klein, J., Figueroa, F., and Klein, D.: H-2 haplotypes, genes, and antigens: second listing. I. Non-H-2 loci on chromosome 17.Immunogenetics 16: 285–317, 1982b

    Google Scholar 

  • Lane, P. W. and Lyon, M. F.: Congenic and segregating inbred strains. Mutant genes and biochemical loci.In M. F. Lyon and A. G. Searle (eds.):Genetic Variants and Strains of the Laboratory Mouse, 2nd edn. pp. 825–842, Oxford University Press, Oxford, 1989

    Google Scholar 

  • Morse, H. C. (ed.):Origins of Inbred Mice, Academic Press, New York, 1978

    Google Scholar 

  • Prehn, R. T. and Main, J. M.: Number of mouse histocompatibility genes involved in skin grafting from strain BALB/cAn to strain DBA/2.J Natl Cancer Inst 20: 207–209, 1958

    Google Scholar 

  • Röhme, D., Fox, H., Herrmann, B., Frischauf, A.-M., Edström, J.-E., Mains, P., Silver, L. M., and Lehrach, H.: Molecular clones of the mouset complex derived from microdissected metaphase chromosomes.Cell 36: 783–788, 1984

    Google Scholar 

  • Snell, G. D.: Methods for the study of histocompatibility genes.J Genetics 49: 87–108, 1948

    Google Scholar 

  • Snell, G. D.: The genetics of transplantation.J Natl Cancer Inst 14: 691–700, 1953

    Google Scholar 

  • Vincek, V., Kawaguchi, H., Mizuno, K., Zaleska-Rutczynska, Z., Kasahara, M., Forejt, J., Figueroa, F., and Klein, J.: Linkage map of mouse chromosome 17: localization of 27 new DNA markers.Genomics 5: 773–786, 1989

    Google Scholar 

  • Zaleska-Rutczynska, Z. and Klein, J.: Histocompatibility-2 system in wild mice. V. Serological analysis of sixteen B10.W congenic lines.J Immunol 119: 1903–1911, 1977

    Google Scholar 

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Vincek, V., Sertić, J., Zaleska-Rutczynska, Z. et al. Characterization ofH-2 congenic strains using DNA markers. Immunogenetics 31, 45–51 (1990). https://doi.org/10.1007/BF00702488

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