Summary
Electron microscopic observations are reported on a case of progressive multifocal leukoencephalopathy unassociated with any lympho-proliferative disease. The morphological evidence suggests that the condition may be caused by the replication, in the glial cell nuclei of the central nervous system, of an infective agent belonging, or related, to the papova group of viruses. The possible implications of these observations are briefly discussed.
Résumé
Un cas de leucoencéphalopathie multifocale progressive, qui n'était associé à aucune prolifération du système lymphatique, a été étudié en microscopie électronique. Les observations parlent en faveur d'un processus dont l'origine semble résider dans la multiplication d'un agent infectieux dans les noyaux des cellules gliales du système nerveux central. Les particules étudiées présentent des caractères morphologiques qui les apparentent aux membres du group des virus papova (papillome, polyome, agent vacuolant SV-40). La signification de ces observations est brièvement discutée.
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References
Åström, K. E., E. L. Mancall, andE. P. Richardson jr.: Progressive multifocal leukoencephalopathy. Brain81, 93–111 (1958).
Bennett, H. S., andJ. H. Luft: S-Collidine as a basis for buffering fixatives. J. biophys. biochem. Cytol.6, 113–114 (1959).
Bethlem, J., J. van Gool, andW. A. den Hartog Jager: Progressive multifocal leucoencephalopathy associated with multiple myeloma. Acta neuropath. (Berl.)3, 525–528 (1964).
Cavanagh, J. B., D. Greenbaum A. H. E. Marshall, andL. J. Rubinstein: Cerebral demyelination associated with disorders of the reticuloendothelial system. Lancet1959 II, 524–529.
Chandor, S. B., L. S. Forno andN. A. Wivel: Progressive multifocal leucoencephalopathy: A report of two cases. J. Neurol. Neurosurg. Psychiat.28, 260–271 (1965).
D'Agostino, A. N., G. L. Pease, andJ. W. Kernohan: Cerebral demyelination associated woth polycythemia vera. J. Neuropath. exp. Neurol.22, 138–147 (1963).
Deep, W. D., J. F. Fraumeni, C. K. Tashima, andR. McDivitt: Leukoencephalopathy and dermatomyositis in Hodgkin's disease. A case report. Arch. intern. Med.113, 635–640 (1964).
Dolman, C. L., andA. R. M. Cairns: Leukoencephalopathy associated with Hodgkin's disease. Neurology (Minneap.)11, 349–353 (1961).
Greenfield, J. G. andA. Meyer: General pathology of the nerve cell and neuroglia. In:Greenfield's Neuropathology, Chap. 1, p. 55, 2nd edition. London, E. Arnold 1963.
Headington, J. T., andW. O. Umiker: Progressive multifocal leukoencephalopathy. A case report. Neurology (Minneap.)12, 434–439 (1962).
Horne, R. W. andP. Wildy: Virus structure revealed by negative staining. Advanc. Virus Res.10, 101–170 (1963).
Lapham, L. W., M. A. Johnstone, andK. H. Brundjar: A new paraffin method for the combined staining of myelin and glial fibers. J. Neuropath. exp. Neurol.23, 156–160 (1964).
Lendrum, A. C.: Phloxin-tartrazine method as general histological stain and for demonstration of inclusion bodies. J. Path. Bact.59, 399–404 (1947).
Melnick, J. L.: Papova virus group. Science135, 1128–1130 (1962).
Mollenhauer, H. H.: Plastic embedding mixtures for use in electron microscopy. Stain Technol.39, 111–114 (1964).
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–212 (1963).
Richardson, E. P., Jr.: Progressive multifocal leukoencephalopathy. New Engl. J. Med.265, 815–823 (1961).
Sabatini, D. D., K. Bensch andR. J. Barrnett: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol.17, 19–58 (1963).
Smith, J. L.: Progressive multifocal leukoencephalopathy. Arch. Ophthal.62, 828–832 (1959).
Terry, R. D.: Personal communication (1963).
Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol.4, 475–478 (1958).
Weinstein, V. F., A. L. Woolf andM. J. Meynell: Progressive multifocal leucoencephalopathy and primary hyperplenism. With a note on the association between disease of the reticuloendothelial system and progressive multifocal leucoencephalopathy. J. clin. Path.16, 405–418 (1963).
Wilner, B. I.: Classification of the major groups of human and lower animal viruses. p. 25, 2nd edition. Berkeley, Calif.: Cutter Laboratories 1964.
Zu Rhein, G. M., andS. M. Chou: Particles resembling papova viruses in human cerebral demyelinating disease. Science148, 1477–1479 (1965).
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This work was supported in part by Research Grants HE 06716 and GM 09227 from the National Institutes of Health, U.S. Public Health Service.
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Silverman, L., Rubinstein, L.J. Electron microscopic observations on a case of progressive multifocal leukoencephalopathy. Acta Neuropathol 5, 215–224 (1965). https://doi.org/10.1007/BF00686519
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DOI: https://doi.org/10.1007/BF00686519