Abstract
The molecular weights ofwaxy proteins, by SDS-PAGE, and the N-terminal amino acid sequences of mature protein and of V8 protease-induced fragments were determined in diploid cereals. The homology of the primary structure was relatively high among cereals examined here, and there appeared to be a common sequence, V-F-V-G-A-E-M-A, in the vicinity of the N terminus. Based on the amino acid sequences, these cereals could be divided into two groups, including corn and rice in one and diploid wheat, fourAegilops species, rye, and barley in the other. In diploid wheat andAegilops species there were substitutions of amino acids in the primary structure. Variations of this sort suggest that the primary structure ofwaxy proteins would provide clues to the phylogenetic relations in the wheat group.
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Taira, T., Fujita, N., Takaoka, K. et al. Variation in the primary structure ofwaxy proteins (granule-bound starch synthase) in diploid cereals. Biochem Genet 33, 269–281 (1995). https://doi.org/10.1007/BF00553625
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DOI: https://doi.org/10.1007/BF00553625