Summary
The influence of systemic treatment with biosynthetic human growth hormone on bone formation was studied, comparing rabbit radial bone defects grafted with demineralized autologous bone with contrallateral defects grafted with a composite including bone marrow. At 14 and 28 days after operation the bone-forming process was evaluated by roentgenography (planimetry included), and by scintigraphy after injection of 99mTc-labeled dicarboxypropane diphosphonate. At 28 days autoradiography was also performed. The roentgenographic and scintigraphic examinations revealed no difference between the grafted sides at either 14 or 28 days postoperatively. The elimination, after 14 days' growth hormone treatment, of a previously found difference between the marrow- and the nonmarrow-grafted sides might be an indication of accelerated osteogenesis at the beginning of the healing process.
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References
Ahl T, Kaléen R (1979) Effect of human growth hormone in the treatment of pseudarthrosis. Opuscula Medica 1:26
Burwell RG (1964) Studies in the transplantation of bone. VII. The fresh composite homograft-autograft of cancellous bone; an anlysis of factors leading to osteogenesis in marrow transplants and marrow-containing grafts. J Bone Joint Surg [Br] 46:110
Daughaday WH, Reeder S (1966) Synchronous activation of DNA synthesis in hypophysectomized rat cartilage by growth hormone. J Lab Clin Med 68:357
Eden S, Isaksson OPG, Madsen K, Friberg W (1983) Specific binding of growth hormone to isolated chondrocytes from rabbit ear and epiphyseal plate. Endocrinology
Frost HM (1962) A model of endocrine control of bone remodeling. Henry Ford Hosp Med Bull 10 (2):119
Geschwind II (1966) Species specificity of anterior pituitary hormones. In: Harris GW, Donovan BT (eds) The pituitary gland, vol 2. Butterworths, London, pp 589–612
Handelsman MB, Gordon EF (1930) Growth and bone changes in rats injected with anterior pituitary extract. J Pharmacol Exp Ther 38:349
Harris JA, Bean DA, Banks HH (1975) Effect of phosphate supplementation, thyrocalcitonin and growth hormone on strength of fracture healing. Surgical Forum 26:519
Harris WH, Heaney RP, Jowsey J, Cockin J, Akins C, Grahamn J, Weinberg EH (1972) Growth hormone: the effect on skeletal renewal in adult dog. Calcif Tissue Res 10:1
Isaksson OGP, Jansson J-O, Gause IAM (1982) Growth hormone stimulates longitudinal bone growth directly. Science 216:1237
Koskinen EVS, Lindholm RV, Nieminen RA, Puranen JP, Attila W (1978) Human growth hormone in delayed union and non-union of fractures. Int Orthop 1:317
Kostyo JL, Isaksson O (1977) Growth hormone and the regulation of somatic growth. In: Greep RO (ed) International review of physiology. Reproductive physiology II, vol 13. Baltimore, pp 255–274
Lindholm RV, Koskinen EVS, Puranen J, Nieminen RA, Kairaluoma M, Attila W (1977) Human growth hormone in the treatment of fresh fractures. Horm Metab Res 9:245
Mankin HJ, Trasher AZ, Weinberg EH, Harris WH (1978) Dissociation between the effect of bovine growth hormone in articular cartilage and in bone of the adult dog. J Bone Joint Surg [Am] 60:1071
Northmore-Ball MD, Wood MR, Meggitt BF (1980) A biomechanical study of the effects of growth hormone in experimental fracture healing. J Bone Joint Surg [Br] 62:391
Reddi AH, Sullivan NS (1980) Matrix-induced endochondral bone differentiation: influence of hypophysectomy, growth hormone, and thyroid-stimulating hormone. Endocrinology 107:1291
Thorngren K-G, Hansson LI (1973) Cell kinetics of the growth plate in hypophysectomized rats treated with growth hormone and thyroxine. Z Zellforsch 142:431–442
Thorngren K-G, Hansson LI, Menander-Sellman K, Stenström A (1973) Effect of dose and administration period of growth hormone on longitudinal bone growth. Acta Endocrinol 74:1–23
Skottner A, Forsman A, Löfberg E, Thorngren K-G Comparison of methionyl human growth hormone and pituitary growth hormone on somatic growth of hypophysectomized rats. Acta Endocrinol (Copenh) 107:231–234
Urist MR (1972) Growth hormone and skeletal tissue metabolism. In: Bourne GH (ed) Biochemistry and physiology of bone, vol II. Academic Press, New York, pp 155–194
Urist MR (1980) Bone transplants and implants. In: Urist MR (ed) Fundamental and clinical bone physiology. Lippincott, Philadelphia, pp 331–368
Urist MR, De Lange RJ, Finerman GAM (1983) Bone cell differentiation and growth factors. Science 220:680–686
Wittbjer J, Palmer B, Rohlin M, Thorngren K-G (1982) Osteogenetic activity in composite grafts of demineralized compact bone and marrow. Clin Orthop 173:229–238
Wittbjer J, Rohlin M, Thorngren K-G (1983) Bone formation in demineralized bone transplants treated with biosynthetic human growth hormone. Scand J Plast Reconstr Surg 17:109–117
Zadeck RE, Robinson RA (1961) The effect of growth hormone on healing of an experimental long-bone defect. J Bone Joint Surg [Am] 43:1261
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Wittbjer, J., Aspenberg, P. & Thorngren, K.G. Composite grafts of demineralized compact bone and marrow under systemic biosynthetic growth hormone treatment. Arch. Orth. Traum. Surg. 104, 233–237 (1985). https://doi.org/10.1007/BF00450216
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DOI: https://doi.org/10.1007/BF00450216