Summary
A linear 11.3 kb plasmid occurs in preparations of mitochondrial DNA from certainBrassica species and cultivars. Evidence that protein is associated with the ends of the plasmid was obtained in this study. Plasmid DNA ran as a distinct band during electrophoresis only if it had been treated with proteinase K. The hybridization patterns of clones from different regions of the plasmid to restriction digests of the protein-DNA complex revealed that the protein is associated with the end fragments of the plasmid. Exonuclease treatments indicated that the 5′ ends of the plasmid DNA molecule are blocked but that the 3′ ends are free.
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References
Carusi EA (1977) Virology 71:380–394
Esser K, Kuck U, Stahl U, Tudzynski P (1983) Curr Genet 7:239–243
Ferris PJ, Vogt VM (1982) J Mol Biol 159:359–381
Gendel S, Straus N, Pulleyblank D, Williams J (1983) J Bacteriol 156:148–154
Hirochika H, Sakaguchi K (1982) Plasmid 7:59–65
Kemble RJ, Thompson RD (1982) Nucleic Acids Res 10:8181–8190
Kolodner R, Tewari KK (1972) Proc Natl Acad Sci USA 69:1830–1834
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Nature (London) 301:725–728
Rekosh DMK, Russel WC, Bellet AJD, Robinson AJ (1977) Cell 11:283–295
Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) J Mol Biol 113:237–251
Salas M, Mellado RP, Vinuela E (1978) J Mol Biol 119:269–291
Stillman BW (1983) Cell 35:7–9
Truett MA, Gall JG (1977) Chromosoma 64:295–303
Vedel F, Mathieu C (1983) Curr Genet 7:13–20
Wahl GM, Stern M, Stark G (1979) Proc Natl Acad Sci USA 76:3683–3867
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Erickson, L., Beversdorf, W.D. & Pauls, K.P. Linear mitochondrial plasmid inBrassica has terminal protein. Curr Genet 9, 679–682 (1985). https://doi.org/10.1007/BF00449821
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DOI: https://doi.org/10.1007/BF00449821