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The glucan-chitin complex in Saccharomyces cerevisiae

V. Precise location of chitin and glucan in bud scar and their physico-chemical characterization

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Abstract

Scar rings (SR) from scarless cells at the early stages of budding and mature bud scars from Saccharomyces cerevisiae isolated by both chemical and enzymic treatment of cell walls were observed by selected-area electron diffraction (SAED), X-ray diffraction and electron microscopy with simultaneous physico-chemical characterization (including molar mass, intrinsic viscosity and crystallite size) of α-chitin and glucan. The SR, composed of glucan with strong 0.608; 0.397 and 0.293 nm X-ray reflections, was formed at the start of budding. The SAED patterns of α-chitin both in the adjacent circular zone and in the parts of newly formed primary septum (PS), observed when the development of the PS started, did not differ from those of α-chitin in the single mature bud scar. The bud scar consisted of α-chitin, glucan and mannan and their content, as well as the crystallite size of chitin, depended on the mode of preparation.

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Abbreviations

ACZ:

Adjacent circular zone

BS:

Birth scar

CFW:

Calcofluor white M2R new: Disodium salt of 4,4′-bis-(4-anilino-bis-diethyl-amino-S-triazine-2-yl-amino)-2,2′-stilbene-disulfonic acid

EDI:

Electron dense istmus

PS:

Primary septum

SAED:

Selected area electron diffraction

SEM:

Scanning electron microscopy

SR:

Scar ring

TEM:

Transmission electron microscopy

XRD:

X-ray diffraction

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Authors and Affiliations

  1. Institute of Microbiology, Czechoslovak Academy of Sciences, Vídeňská 1083, CS-14220, Prague 4, Czechoslovakia

    Zdeněk Holan, Vladimír Pokorný & Karel Beran

  2. Institute of Physics, Czechoslovak Academy of Sciences, Na Slovance 2, CS-18040, Prague 8, Czechoslovakia

    Antonín Gemperle

  3. Institute of Macromolecular Chemistry, Czechoslovak Academy of Sciences, Heyrovského nám. 2, CS-16000, Prague 6, Czechoslovakia

    Zdeněk Tuzar

Authors
  1. Zdeněk Holan
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  2. Vladimír Pokorný
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  3. Karel Beran
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  4. Antonín Gemperle
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  5. Zdeněk Tuzar
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  6. Josef Baldrián
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Holan, Z., Pokorný, V., Beran, K. et al. The glucan-chitin complex in Saccharomyces cerevisiae . Arch. Microbiol. 130, 312–318 (1981). https://doi.org/10.1007/BF00425946

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  • DOI: https://doi.org/10.1007/BF00425946

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