Summary
A range of mutants auxotrophic for cysteine (cys) and resistant to selenate (sel) were isolated from many Streptomyces strains but chiefly from S. coelicolor A3(2) and S. lividans 66. Two of the classes of sel/cys mutants probably contained simple biochemical lesions of sulphate permease (selC) and ATP sulphurylase (selA) activities, while a further two classes (selD and selE) were pleiotropic and possibly regulatory. Most classes of sel mutations were clustered around the cysD locus of S. coelicolor. Segments of chromosomal DNA cloned from S. coelicolor, S. cattleya and S. clavuligerus and able to complement various sel/cys mutations allowed the relative positions of these mutations and the cysC and cysD mutations of S. coelicolor to be determined. The sel/cys DNA can be used for two-way selection: Cys+Sels↔Cys-Selr.
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References
Altenbuchner J, Cullum J (1984) DNA amplification and an unstable arginine gene in S. lividans 66. Mol Gen Genet 195:134–138
Arst HN (1968) Genetic analysis of the first steps of sulphate metabolism in A. nidulans. Nature 219:268–270
Bellenger N, Nissen P, Wood TC, Segel IH (1968) Specificity and control of choline-O-sulphate transport in filamentous fungi. J Bacteriol 96:1574–1585
Biro S, Chater KF (1987) Cloning of S. griseus and S. lividans genes for glycerol dissimilation. Gene 56:79–86
Breton A, Surdin-Kerjan Y (1977) Sulfate uptake in Saccharomyces cerevisiae: biochemical and genetic study. J Bacteriol 132:224–232
Chater KF, Bruton CJ (1985) Resistance, regulatory and production genes for the antibiotic methylenomycin are clustered. EMBO J 4:1893–1897
Feitelson JS, Malpartida F, Hopwood DA (1985) Genetic and biochemical characterization of the red gene cluster of S. coelicolor A3(2). J Gen Microbiol 131:2431–2441
Fisher SH, Bruton CJ, Chater KF (1987) The glucose kinase gene of S. coelicolor and its use in selecting spontaneous deletions for desired regions of the genome. Mol Gen Genet 206:35–44
Flavin M (1975) Methionine biosynthesis. In: Greenberg DM (ed) Metabolism of sulphur compounds. Academic Press, New York, p 457
Fornwald JA, Schmidt FJ, Adams CW, Rosenberg M, Brawner ME (1987) Two promoters, one inducible and one constitutive, control transcription of the S. lividans galactose operon. Proc Natl Acad Sci USA 84:2130–2134
Hopwood DA, Chater KF, Dowding JE, Vivian A (1973) Advances in S. coelicolor genetics. Bacteriol Rev 37:371–405
Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM, Schrempf H (1985) Genetic manipulation of streptomyces: a laboratory manual. John Innes Foundation, Norwich
Hulanicka MD, Garrett C, Jagura-Burdzy G, Kredich NM (1986) Cloning and characterization of the cysAMK region of S. typhimurium. J Bacteriol 168:322–327
Ikeda H, Seno ET, Bruton CJ, Chater KF (1984) Genetic mapping, cloning and physiological aspects of the glucose kinase gene of S. coelicolor. Mol Gen Genet 196:501–507
Kendall K, Ali-Dunkrah U, Cullum J (1987) Cloning of the galactokinase gene (galK) from S. coelicolor A3(2). J Gen Microbiol 133:721–725
Kern BA, Inamine E (1981) Cystathionine γ-lyase activity in the cephamycin C producer S. lactamdurans. J Antibiot 34:583–589
Kitano K, Nozaki Y, Imada A (1985) Selective accumulation of unsulfated carbapenem antibiotics by sulfate transport-negative mutants of S. griseus subsp. cryophilus C-19393. Agric Biol Chem 49:677–684
Kredich NM (1971) Regulation of Lxx-cysteine biosynthesis in S. typhimurium. J Biol Chem 246:3474–3484
Leinweber F-J, Monty KJ (1965) Cysteine biosynthesis in N. crassa. J Biol Chem 240:782–787
Lydiate DJ, Ikeda H, Hopwood DA (1986) A 2.6 kb DNA sequence of S. coelicolor A3(2) which functions as a transposable element. Mol Gen Genet 203:79–88
Malpartida F, Hopwood DA (1986) Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin in S. coelicolor A3(2). Mol Gen Genet 205:66–73
Marzluf GA (1970) Genetic and metabolic controls for sulfate metabolism in N. crassa: isolation and study of chromate-resistant and sulfate transport-negative mutants. J Bacteriol 102:716–721
Mendez C, Chater KF (1987) Cloning of whiG, a gene critical for sporulation of S. coelicolor A3(2). J Bacteriol 169:5715–5720
Murray NE, Brammar WJ, Murray K (1977) Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet 150:53–61
Nakamura T, Sato R (1963) Synthesis from sulphate and accumulation of S-sulphocysteine by a mutant strain of A. nidulans. Biochem J 86:328–335
Nakamura T, Iwahashi H, Eguchi Y (1984) Enzymatic proof for the identity of the S-sulfocysteine synthase and the cysteine synthase B of S. typhimurium. J Bacteriol 158:1122–1127
Ohta N, Galsworthy PR, Pradee AB (1971) Genetics of sulfate transport by S. typhimurium. J Bacteriol 105:1053–1062
Piret JM, Chater KF (1985) Phage-mediated cloning of bldA, a region involved in S. coelicolor morphological development, and its analysis by genetic complementation. J Bacteriol 163:965–972
Postgate JR (1963) The examination of sulphur auxotrophs: a warning. J Gen Microbiol 30:481–484
Rodicio MR, Bruton CJ, Chater KF (1985) New derivatives of the Streptomyces temperate phage ϕC31 useful for the cloning and functional analysis of Streptomyces DNA. Gene 34:283–292
Sanderson KE, Roth JR (1983) Linkage map of S. typhimurium, edition VI. Microbiol Rev 47:410–453
Seno ET, Bruton CJ, Chater KF (1984) The glycerol utilization operon of S. coelicolor: genetic mapping of gyl mutations and the analysis of cloned gyl DNA. Mol Gen Genet 193:119–128
Smith CP, Chater KF (1987) Physiology, genetics and molecular biology of glycerol utilization in S. coelicolor. In: Alacevic M, Hranueli D, Toman Z (eds) Genetics of Industrial Micro-organisms, vol B. Pliva, Zagreb, pp 7–15
Ward JM, Janssen GR, Kieser T, Bibb MJ, Buttner MJ, Bibb MJ (1986) Construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator. Mol Gen Genet 203:468–478
Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
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Lydiate, D.J., Mendez, C., Kieser, H.M. et al. Mutation and cloning of clustered Streptomyces genes essential for sulphate metabolism. Molec. Gen. Genet. 211, 415–423 (1988). https://doi.org/10.1007/BF00425694
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DOI: https://doi.org/10.1007/BF00425694