Summary
Conidial protoplasts of an A. nidulans amdS deletion strain (MH1277) have been transformed to the AmdS+ phenotype with a plasmid carrying the wild type gene (p3SR2). Optimalisation of transformation and plating conditions now has resulted in frequencies of 300–400 transformants per μg of DNA.
Analysis of DNA from AmdS+ transformants of MH1277 showed that transformation had occurred by integration of vector DNA sequences into the genome. In virtually all these transformants multiple copies of the vector were present in a tandemly repeated fashion, not preferentially at the resident, partially deleted amdS gene. It is suggested that the observed integration phenomena are dependent on the genetic background of the A. nidulans strain, used for transformation. A model to explain the tandem type of integration is proposed.
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Abbreviations
- bp:
-
base pairs
- kb:
-
1,000 bp
- EtBr:
-
ethidiumbromide
- PEG:
-
polyethyleneglycol
- r-DNA:
-
ribosomal DNA
- c.f.u.:
-
colony forming units
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Wernars, K., Goosen, T., Wennekes, L.M.J. et al. Gene amplification in Aspergillus nidulans by transformation with vectors containing the amdS gene. Curr Genet 9, 361–368 (1985). https://doi.org/10.1007/BF00421606
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DOI: https://doi.org/10.1007/BF00421606