Summary
We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both stationary and exponential phase cells. These and other observations indicate that the mechanisms of repair of alkylation damage and mutagenesis differ markedly between S. cerevisiae and Escherichia coli.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Burke W, Fangman WL (1975) Cell 5:263–269
Calderon IL, Cerda-Olmeda E (1982) Curr Genet 6:237–243
Cerda-Olmeda E, Hanawalt PC, Guerola N (1968) J Mol Biol 33:705–719
Coulondre C, Miller JH (1977) J Mol Biol 117:577–606
Dawes IW, Carter BL (1974) Nature (London) 250:709–712
Donahue TF, Farabaugh PJ, Fink GR (1982) Gene 18:47–59
Duntze W, Stotzler D, Bucking-Throm E, Kalbitzer S (1973) Eur J Biochem 35:357–365
Evensen G, Seeberg E (1982) Nature (London) 296:773–775
Guerola N, Ingraham JL, Cerda-Olmedo E (1971) Nature (London) 230:122–125
Hadden CT, Foote RS, Mitra S (1983) J Bacteriol 153:746–763
Hereford LM, Hartwell LH (1973) Nature (London) 244:129–131
Karran P, Arlett CF, Broughton BC (1982a) Biochimie 64:717–721
Karran P, Hjelmgren T, Lindahl T (1982b) Nature (London) 296:770–773
Kee SG, Haber JE (1975) Proc Natl Acad Sci USA 72:1179–1183
Lindahl T, Demple B, Robins P (1982) EMBO J 1:1359–1365
Lindahl T, Karran P (1983) In: 13th International Cancer Congress, Part B, Biology of Cancer (1). Liss, New York, pp 241–250
Maga JA, McEntee K (1985) Mol Gen Genet 200:313–321
Margison GP (1981) Carcinogenesis 2:431–434
McCarthy TV, Lindahl T (1985) Nucleic Acids Res 13:2683–2698
McCarthy TV, Karran P, Lindahl T (1984) EMBO J 3:545–550
Olsson M, Lindahl T (1980) J Biol Chem 255:10569–10571
Pegg AE, Perry W (1981) Carcinogenesis 2:1195–1200
Prakash L (1974) Genetics 78:1101–1118
Prakash L (1976) Genetics 83:295–301
Prakash L, Higgins D (1982) Carcinogenesis 3:439–444
Prakash L, Sherman F (1973) J Mol Biol 79:65–82
Samson L, Cairns J (1977) Nature (London) 267:281–282
Schendel PF, Robbins PE (1978) Proc Natl Acad Sci USA 75:6017–6020
Sklar R, Strauss B (1980) J Mol Biol 143:343–362
Sklar R, Brady K, Strauss B (1981) Carcinogenesis 2:1293–1298
Swann PF, Mace R (1980) Chem Biol Inter 31:239–245
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Polakowska, R., Perozzi, G. & Prakash, L. Alkylation mutagenesis in Saccharomyces cerevisiae: lack of evidence for an adaptive response. Curr Genet 10, 647–655 (1986). https://doi.org/10.1007/BF00410912
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00410912