Abstract
Abscisic acid (ABA) in extracts of somatic embryos and seeds of Gloryvine (Vitis vinifera L.xV. rupestris Scheele) was measured by gas chromatography-mass spectrometry-selected ion monitoring using deuterated ABA, (±)-[C-3Me-2H3]ABA, ([2H3]ABA) as internal standard. The ABA content increased rapidly during embryogeny (0.035 ng/embryo at the globular stage to 0.22 ng/embryo at the mature stage). The level of ABA in the tissues of somatic embryos, expressed in ng/mg dry weight, decreased from the globular stage (0.76 ng/mg) to the mature stage (0.25 ng/mg). Chilling (4° C) induced normal germination of seeds and mature somatic embryos and precocious germination of globular, heart-shaped and torpedoshaped somatic embryos. In all cases chilling led to a marked reduction in endogenous ABA. Exogenous (±)-ABA inhibited the germination of chilled somatic embryos.
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Abbreviations
- ABA:
-
abscisic acid
- [2H3]ABA:
-
(±)-[C-3Me-2H3]-abscisic acid
- BHT:
-
2,6-di-t-butyl-4-methylphenol
- GC-MS:
-
gas chromatography-mass spectrometry
- Me-ABA and Me-[2H3]ABA:
-
methyl esters of ABA and [2H3]ABA, respectively
- SIM:
-
selected ion monitoring
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Rajasekaran, K., Vine, J. & Mullins, M.G. Dormancy in somatic embryos and seeds ofVitis: changes in endogenous abscisic acid during embryogeny and germination. Planta 154, 139–144 (1982). https://doi.org/10.1007/BF00387907
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DOI: https://doi.org/10.1007/BF00387907