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Does indoleacetic acid promote growth via cell wall acidification?

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Abstract

Growth of Triticum aestivum L. cv. Cappelle Desprez coleoptiles is promoted by 5.7×10−5 M indole acetic acid (IAA) as effectively in pH 3.4 buffer as in water, but IAA is not effective in the presence of buffer at pH 3.0 or 3.2 A combination of 5.7×10−5 M IAA and pH 3.4 buffer promotes growth to a greater extent than pH 3.2 buffer alone, which is optimal for acid-induced growth. IAA employed at 10−7 M is still effective at promoting growth in the presence of pH 3.4 buffer, moreover, IAA at 10−7 M interacts synergistically with the acidic buffer to promote growth. It is concluded that IAA and acid promote growth via separate mechanisms, and that IAA does not promote cell wall loosening by rendering the cell wall more acid.

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Abbreviations

IAA:

Indoleacetic acid

References

  • Burström, H.G.: Wishful thinking of turgor. Nature 234, 488 (1971)

    Google Scholar 

  • Cleland, R.: Auxin-induced hydrogen ion secretion from Avena coleoptiles. Proc. Natl. Acad. Sci. USA 70, 3092–3093 (1973)

    Google Scholar 

  • Cleland, R.: Auxin-induced hydrogen-ion excretion: correlation with growth and control by external pH and water stress. Planta 127, 233–242 (1975)

    Google Scholar 

  • Cleland, R. Kinetics of hormone-induced H+ secretion. Plant Physiol. 58, 210–213 (1976)

    Google Scholar 

  • Evans, M.L., Ray, P.M.: Timing of the auxin response in coleoptiles and its implications regarding auxin action. J. gen. Physiol. 53, 1–20 (1969)

    Google Scholar 

  • Hager, A., Menzel, H., Krauss, A.: Versuche und Hypothese zur Primärwirkung des Auxins beim Streckungswachstum. Planta 100, 47–75 (1971)

    Google Scholar 

  • Heath, O.V.S., Clark, J.E.: Chelating agents as plant growth substances. Nature 177, 1118–1121 (1956)

    Google Scholar 

  • Ilan, I.: On auxin-induced pH drop and on the improbability of its involvement in the primary mechanism of auxin-induced growth promotion. Physiol. Plant. 28, 146–148 (1973)

    Google Scholar 

  • Jacobs, M., Ray, P.M.: Rapid auxin-induced decrease in free space pH and its relationship to auxin-induced growth in maize and pea. Plant Physiol. 58, 203–209 (1976)

    Google Scholar 

  • Keegstra, K., Talmadge, K.W., Bauer, W.D., Albersheim, P.: The structure of plant cell walls. III A model of the walls of suspension-cultured sycamore cells based on the interconnections of the macromolecular components. Plant Physiol. 51, 188–196 (1973)

    Google Scholar 

  • Lockhart, J.A.: Physical nature of irreversible deformation of plant cells. Plant Physiol. 42, 1545–1552 (1967)

    Google Scholar 

  • Marrè, E., Lado, P., Rasi-Caldogno, F., Colombo, R.: Correlation between cell enlargement in pea internode segments and decrease in the pH of the medium of incubation. I Effects of fusicoccin, natural and synthetic auxins, and mannitol. Plant Sci. Lett. 1, 179–184 (1973)

    Google Scholar 

  • Parrish, D.J., Davies, P.J.: On the relationships between extracellular pH and the growth of excised pea stem segments. Plant Physiol. 59, 574–578 (1977)

    Google Scholar 

  • Penny, P., Dunlop, J., Perley, J.E., Penny, D.: pH and auxin-induced growth: A causal relationship? Plant Sci. Lett. 4, 35–40 (1975)

    Google Scholar 

  • Perley, J.E., Penny, D., Penny, P.: A difference between auxin-induced and hydrogen-ion induced growth. Plant. Sci. Lett. 4, 133–136 (1975)

    Google Scholar 

  • Pope, D.G.: Separation of Indol-3-ylacetic acid-induced growth from acid-induced growth in Avena coleoptiles.: Ann. Bot. 41, 1069–1071 (1977)

    Google Scholar 

  • Ray, P.M.: Auxin-binding sites of maize coleoptiles are localized on membranes of the endoplasmic reticulum. Plant Physiol. 59, 594–599 (1977)

    Google Scholar 

  • Rayle, D.L., Cleland, R.: Enhancement of wall loosening and elongation by acid solution. Plant Physiol. 46, 250–253 (1970)

    Google Scholar 

  • Rayle, D.L., Cleland, R.: The in vitro acid-growth response: relation to in vivo growth responses and auxin action. Planta 104, 282–296 (1972)

    Google Scholar 

  • Rayle, D.L.: Auxin-induced hydrogen-ion secretion in Avena coleoptiles and its implications. Planta 114, 63–73 (1973)

    Google Scholar 

  • Rubery, P.H., Sheldrake, A.R.: Effect of pH and surface charge on cell uptake of auxin. Nature New Biol. 244, 285–288 (1973)

    Google Scholar 

  • Tagawa, T., Bonner, J.: Mechanical properties of the Avena coleoptile as related to auxin and ionic interactions. Plant Physiol. 32, 207–212 (1957)

    Google Scholar 

  • Vanderhoef, L.N., Findley, J.S., Burke, J.J., Blizzard, W.E.: Auxin has no effect on modification of external pH by soybean hypocotyl cells. Plant Physiol. 59, 1000–1003 (1977)

    Google Scholar 

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Pope, D.G. Does indoleacetic acid promote growth via cell wall acidification?. Planta 140, 137–142 (1978). https://doi.org/10.1007/BF00384912

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  • DOI: https://doi.org/10.1007/BF00384912

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