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Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene

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Summary

Insertion mutations in yeast caused by the transposable element Ty have been identified at several genetic loci. In all cases so far, the site of Ty insertion has been in the 5′ non-coding region of the affected gene. Experiments presented here demonstrate that the ura3-52 mutation, a non-reverting ura3 mutation, is caused by a Ty insertion mutation within the coding region of the URA3 gene. This Ty insertion within a gene has a similar structure to those in non-coding regions.

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References

  • Botstein D, Falco SC, Stewart SE, Brennan M, Scherer S, Stinchcomb DT, Struhl K, Davis RW (1979) Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments. Gene 8:17–24

    PubMed  Google Scholar 

  • Donahue TF, Daves RS, Lucchini G, Fink GR (1983) A short nucleotide sequence required for regulation of HIS4 by the general control system of yeast. Cell 32:89–98

    Google Scholar 

  • Farabaugh PJ, Fink GR (1980) Insertion of the eukaryotic transposable element Ty1 creates a 5 base pair duplication. Nature 286:352–356

    PubMed  Google Scholar 

  • Jackson JA (1983) Recombination between duplicated genetic elements in yeast. PhD thesis, Cornell University

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. (A laboratory manual). Cold Spring Harbor Laboratory, CSH, New York, pp 545

    Google Scholar 

  • Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. In: Grossman L, Moldaye K (eds) Methods in enzymology, vol 65, Academic Press, New York, pp 499–560

    Google Scholar 

  • Roeder GS, Farabaugh PJ, Chaleff DT, Fink GR (1980) The origins of gene instability in yeast. Science 209:1375–1380

    Google Scholar 

  • Roeder GS, Fink GR (1980) DNA rearrangements associated with a transposable element in yeast. Cell 21:239–249

    Google Scholar 

  • Roeder GS, Fink GR (1982) Transposable elements in yeast. In Shapiro JA (ed) Mobile genetic elements. Academic Press, New York, pp 299–328

    Google Scholar 

  • Rose M (1982) lacZ gene fusion: an assay for expression in yeast. PhD thesis. Massachusetts Institute of Technology

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    Google Scholar 

  • Winston F, Chumley F, Fink GR (1983) Eviction and transplacement of mutant genes in yeast. In: Wu R, Grossman L, Moldave K (eds) Methods in enzymology: Recombinant DNA, Part B. Academic Press, New York, pp 211–228

    Google Scholar 

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Communicated by G.R. Fink

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Rose, M., Winston, F. Identification of a Ty insertion within the coding sequence of the S. cerevisiae URA3 gene. Molec. Gen. Genet. 193, 557–560 (1984). https://doi.org/10.1007/BF00382100

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  • DOI: https://doi.org/10.1007/BF00382100

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