Summary
The sequence of a 6.24 kb DNA segment of the mitochondrial genome from rat liver has been determined. It comprises several genes coding for mitochondrial protein subunits and five tRNA genes in the following order: cytochrome oxidase subunit I — tRNA Ser(UCN) —tRNAAsp — cytochrome oxidase subunit II — tRNALys —ATPase subunit — cytochrome oxidase subunit III —tRNAGly — potential open reading frame — tRNAArg —two potential open reading frames. The tRNA genes were detected by a computer search programme. The assignments for the protein coding sequences were made through comparison with known sequences, mainly from the yeast mitochondrial proteins (e.g. Bonitz et al. 1980). Our data are discussed with regard to the features of gene arrangement, codon usage, and tRNA structure in mammalian mitochondria (Anderson et al. 1981).
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Abbreviations
- COX I, COX II, COX III:
-
mitochondrial cytochrome oxidase subunits I, II, and III
- ATPase:
-
mitochondrial ATPase subunit 6
- U.R.F.:
-
unidentified reading frame
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(Anderson et al. 1981). Other abbreviations follow IUB-IUPAC conventions.
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Grosskopf, R., Feldmann, H. Analysis of a DNA segment from rat liver mitochondria containing the genes for the cytochrome oxidase subunits I, II and III, ATPase subunit 6, and several tRNA genes. Current Genetics 4, 151–158 (1981). https://doi.org/10.1007/BF00365694
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DOI: https://doi.org/10.1007/BF00365694