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Generation and characterization of an ordered lambda clone array for the 460-kb region surrounding the murine Xist sequence

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Abstract

The Xist sequence has several characteristics that make it a potential candidate for the X-inactivation center. To investigate the role of Xist and adjacent sequences lying within the X-inactivation center candidate region, a 460-kb region surrounding the murine Xist sequence has been arrayed in lambda contigs with a combination of IRS-PCR-based hybridization and YAC fragmentation. The orientation of the Xist sequence in relation to the telomere and centromere of the X Chromosome (Chr) has been established with this contig and shown to be inverted compared to that in human.

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Rougeulle, C., Colleaux, L., Dujon, B. et al. Generation and characterization of an ordered lambda clone array for the 460-kb region surrounding the murine Xist sequence. Mammalian Genome 5, 416–423 (1994). https://doi.org/10.1007/BF00357001

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  • DOI: https://doi.org/10.1007/BF00357001

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