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Meiotic mapping of murine chromosome 17: The string of loci around l(17)-2Pas

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Abstract

We describe a genetic analysis of l(17)-2Pas, an embryonic lethal mutation on murine chromosome 17. Males transmitted the l(17)-2 allele to only 38% of their offspring, whereas females transmitted this allele at 50%. Two-point crosses revealed tight linkage between l(17)-2 and brachyury (T), and deletion mapping placed l(17)-2 outside of the hairpin-tail deletion (T hp). To map this mutation more precisely, we intercrossed hybrid mice that carry distinct alleles at many classical and DNA loci on chromosome 17 and obtained 172 animals recombinant in the T to H-2 region. Strong positive interference was observed over the 14 cM interval from T to H-2K. Thus, a single recombinant can be informative; one such recombinant places l(17)-2 distal of the molecular marker D17Leh66D. Robust genetic maps can be constructed with multilocus crosses that share anchor loci. DNA markers can be interpolated onto these maps retrospectively.

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  1. Thomas R. King

    Present address: The Jackson Laboratory, 600 Main Street, 04609, Bar Harbor, Maine, USA

Authors and Affiliations

  1. Laboratory of Genetics and McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, 53706, Madison, Wisconsin, USA

    Thomas R. King, William F. Dove & Alexandra Shedlovsky

  2. Génétique des Mammifères, Institut Pasteur, 25 rue du Docteur Roux, 75015, Paris, France

    Jean-Louis Guénet

  3. Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35, D-7400, Tübingen 1, West Germany

    Bernhard G. Herrmann

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King, T.R., Dove, W.F., Guénet, JL. et al. Meiotic mapping of murine chromosome 17: The string of loci around l(17)-2Pas . Mammalian Genome 1, 37–46 (1991). https://doi.org/10.1007/BF00350844

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