Summary
We recently reported that the gene for chloroplast tRNACys(GCA) is a pseudogene in the plastid DNA of Epifagus virginiana, a non-photosynthetic parastic flowering plant in the family Orobanchaceae. Since this is the only tRNACys gene in the plastid genome, and since Epifagus appears to possess a functional plastid translational apparatus, it seems probable that nuclear-encoded tRNAs are imported into plastids to effect translation. In this study we have surveyed species closely related to Epifagus to establish how widespread the loss of this tRNA gene has been. We find that Conopholis americana, another non-photosynthetic parasite, lacks the gene altogether, but that seven closely-related photosythetic plants (both parasitic and free-living) maintain an intact chloroplast tRNACys gene. Thus, the tRNACys gene appears to have become non-functional at the same time that photosynthetic ability was lost. This may be because the levels of putatively imported tRNAs are sufficient to meet the demands of plastid gene expression under nonphotosynthetic conditions only.
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dePamphilis CW, Palmer JD (1990) Nature 348:337–339
Doyle JJ, Doyle JL (1987) Phytochem Bull 19:11–15
Hancock K, Hajduk SL (1990) J Biol Chem 265:19208–19215
Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun C-R, Meng B-Y, Li Y-Q, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M (1989) Mol Gen Genet 217:185–194
Hudson GS, Mason JG, Holton TA, Koller B, Cox GB, Whitfeld PR, Bottomley W (1987) J Mol Biol 196:283–298
Kuhsel M, Strickland R, Palmer JD (1990) Science 250:1570–1573
Marechal-Drouard L, Guillemaut P, Cosset A, Arbogast M, Weber F, Weil J-H, Dietrich A (1990) Nucleic Acids Res 18:3689–3696
Morden CW, Wolfe KH, dePamphilis CW, Palmer JD (1991) EMBO J 10:3281–3288
Ozeki H, Ohyama K, Inokuchi H, Fukuzawa H, Kohchi T,Sano T, Nakahigashi K, Umesono K (1987) Cold Spring Harbor Symp Quant Biol 52:791–804
Palmer JD (1986) Methods Enzymol 118:167–186
Pfitzinger H, Guillemaut P, Weil J-H, Pillay DTN (1987) Nucleic Acids Res 15:1377–1386
Pfitzinger H, Weil JH, Pillay DTN, Guillemaut P (1990) Plant Mol Biol 14:805–814
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Shinozaki K, Ohme M, Tanaka T, Wakasugi T Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Sugiura M, Shinozaki K, Zaita N, Kusuda M, Kumano M (1986) Plant Sci 44:211–216
Theriet JW (1967) SIDA 3:87–106
Wakasugi T, Ohme M, Shinozaki K, Sugiura M (1986) Plant Mol Biol 7:385–392
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Communicated by C. W. Birky, Jr.
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Taylor, G.W., Wolfe, K.H., Morden, C.W. et al. Lack of a functional plastid tRNACys gene is associated with loss of photosynthesis in a lineage of parasitic plants. Curr Genet 20, 515–518 (1991). https://doi.org/10.1007/BF00334780
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DOI: https://doi.org/10.1007/BF00334780