Skip to main content
SpringerLink
Log in

Determination of PCBs in biological samples using on-line SFE-GC

  • Original Papers
  • Supercritical Fluid Extraction
  • Published:
Fresenius' Journal of Analytical Chemistry Aims and scope Submit manuscript

Summary

Supercritical fluid extraction (SFE) directly coupled to capillary gas chromatography (GC) has been utilized for selective extraction of polychlorinated biphenyls (PCBs) from the lipid matrix in different biological species using basic alumina as a selective adsorbent. Recoveries were between 70 and 96% and the reproducibilities for all samples were between 3 and 6% relative standard deviation. On-line SFE-GC reduces the potential for sample contamination and loss of analyte as no sample handling or concentration procedures are needed between extraction and analysis. The method has been applied to samples with large differences in fat content and the fat content did not seem to influence the performance of the analysis. Solute trapping, choice of restrictor, extraction volume and detection limit are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Onuska FI, Terry KA (1989) HRC & CC 12:527

    Google Scholar 

  2. King JW, Johnson JH, Friedrich JP (1989) J Agric Food Chem 37:951

    Google Scholar 

  3. Lopez-Avila V, Dodhiwala NS, Beckert WF (1990) J Chromatogr Sci 28:468

    Google Scholar 

  4. Yamaguchi K, Murakami M, Nakano H, Konosu S, Kokura T, Yamamoto H, Kosaka M, Hata K (1986) J Agric Food Chem 34:904

    Google Scholar 

  5. Hawthorne SB, Miller DJ, Krieger MS (1988) Fresenius Z Anal Chem 330:211

    Google Scholar 

  6. Rizvi SSH, Benado AL, Zollweg JA, Daniels JA (1986) Food Technology:55

  7. Lee ML, Markides KE (1990) Analytical supercritical fluid chromatography and extraction. Utah, Chapter 5

  8. Nam KS, Kapila S, Viswanath DS, Clevenger TE, Johansson J, Yanders AF (1989) Chemosphere 19:33

    Google Scholar 

  9. King JW (1989) J Chromatogr Sci 27:355

    Google Scholar 

  10. Murugaverl B, Voorhees KJ (1991) J Microcol Sep 3:11

    Google Scholar 

  11. Greibrokk T, Radke M, Skurdal M, Willsch H (1992) Organic Geochem (in press)

  12. Hawthorne SB, Miller DJ (1989) J Chromatogr 403:63

    Google Scholar 

  13. Raymer JH, Velez GR (1991) J Chromatogr Sci 29:467

    Google Scholar 

  14. Hawthorne SB, Miller DJ, Krieger MS (1989) J Chromatogr Sci 27:347

    Google Scholar 

  15. Nielen MWF, Stab JA, Lingeman H, Brinkman UAT (1991) Chromatographia 32:543

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, University of Oslo, P. O. Box 1033, Blindern, 0315, Oslo, Norway

    Hege Rebecka Johansen & Tyge Greibrokk

  2. Department of Environmental Medicine, National Institute of Public Health, 0462, Oslo, Norway

    Georg Becher

Authors
  1. Hege Rebecka Johansen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Georg Becher
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tyge Greibrokk
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Dedicated to Prof. Dr. Ernst F. G. Klesper on the occasion of his 65th birthday

Rights and permissions

Reprints and permissions

About this article

Cite this article

Johansen, H.R., Becher, G. & Greibrokk, T. Determination of PCBs in biological samples using on-line SFE-GC. Fresenius J Anal Chem 344, 486–491 (1992). https://doi.org/10.1007/BF00323748

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00323748

Keywords

Search

Navigation