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Thin-layer chromatography of glucose and sorbitol on Cu(II)-impregnated silica gel plates

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Abstract

A thin-layer chromatographic (TLC) separation of glucose and sorbitol on Cu(II)-impregnated silica gel plates with n-propanol: water (4:1) v/v as developer and potassium permanganate as detecting reagent has been worked out. The new impregnant is completely insoluble in water and thus enables the use of an aqueous developer. The Rf-values are 55 and 10 for glucose and sorbitol, respectively.

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Authors and Affiliations

  1. Ružer Bošković Institute, P. O. B. 1016, 41001, Zagreb, Croatia

    O. Hadžija & B. Špoljar

  2. Institute of Immunology, Zagreb, Croatia

    Lj. Sesartić

Authors
  1. O. Hadžija
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  2. B. Špoljar
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  3. Lj. Sesartić
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Hadžija, O., Špoljar, B. & Sesartić, L. Thin-layer chromatography of glucose and sorbitol on Cu(II)-impregnated silica gel plates. Fresenius J Anal Chem 348, 782 (1994). https://doi.org/10.1007/BF00323709

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  • DOI: https://doi.org/10.1007/BF00323709

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