Abstract
Using a well characterized anti-serum, the distribution of octopamine-like immunoreactive neurones is described in the locust seventh abdominal (A7) and terminal ganglia (TG), which are associated with genital organs. Apart from 4 paired ventral somata occasionally observed in the TG, all labelled cells could be identified as efferent dorsal- and ventral unpaired median (DUM/VUM) neurones by virtue of the characteristic large size and position of their somata, projections of their primary neurites in DUM-cell tracts, and bifurcating axons which arise from dorsal T-junctions and enter peripheral nerves. For the examined ganglia our data indicate that the whole population of efferent DUM and VUM-cells, defined here as progeny of the segment specific unpaired median neuroblast with peripheral axons, are octopaminergic, and that equal numbers of these cells occur in both sexes: 8 in A7 and 11 in TG. Sex-specific differences are probably restricted to the axonal projections of 5 octopamine-like immunoreactive DUM-somata in A7, and 5 in TG, which in females project into their segment specific sternal nerves, but in males into the genital nerve of the TG. Numerous intersegmentally projecting octopamine-like immunoreactive fibres traverse both ganglia. The majority probably stem from previously described octopamine-like immunoreactive neurones in the thoracic and suboesophageal ganglia.
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Agricola H, Hetel W, Penzlin H (1988) Octopamin — Neurotransmitter, Neuromodulator und Neurohormon. Zool Jb Physiol 92:1–45
Agricola H, Schildberger K, Schmidt A, Naumann W, Reißmann S, Huber F, Penzlin H (1992) Imunocytochemical distribution of allatostatin in the nervous system of the cockroach Periplaneta americana. In: Elsner N, Richter DW (eds) Rhythmogenesis in neurones and networks. Proceedings of the 20th Göttingen Neurobiology Conference. Thieme, Stuttgart New York, p 494
Bate M, Goodman CS, Spitzer NC (1981) Embryonic development of identified neurones: Segment specific differences in the cell homologues. J Neurosci 1:103–106
Bräunig P (1991) Suboesophageal DUM neurons innervate the principal neuropiles of the locust brain. Philos Trans R Soc Lond [Biol] 332:221–240
Doc CD, Goodman CS (1985) Early events in insect neurogenesis I. Development and segmental differences in the pattern of neuronal precursor cells. Dev Biol 111:193–205
Eckert M, Varanka I, Benedecky I (1989) Proctolinergic innervation of the locust oviduct. Zool Jb Physiol 93:471–479
Eckert M, Rapus J, Nürnberger A, Penzlin H (1992) A new specific antibody reveals octopamine-like immunoreactivity in cockroach ventral nerve cord. J Comp Neurol 322:1–15
Evans PD (1985) Octopamine. In: Kerkut GA, Gilbert LI (eds) Comprehensive insect physiology, biochemistry and pharmacology. Pergamon Press, Oxford, pp 499–530
Evans PD, O'Shea M (1978) The identification of an octopaminergic neurone and the modulation of a myogenic rhythm in the locust. J Exp Biol 73:235–260
Falck B, Hillap N-A, Thieme G, Torp A (1962) Fluorescence of catacholamines and related compounds condensed with formaldehyde. J Histochem Cytochem 10:348–354
Ferber M, Pflügr H-J (1990) Bilaterally projecting neurones in pregenital abdominal ganglia of the locust: anatomy and periheral targets. J Comp Neurol 302:447–460
Ferber M, Pflüger H-J (1992) An identified dorsal unpaired median neurone and bilaterally projecting neurones exhibiting bovine pancreatic polypeptide-like/FMRFamide-like immunoreactivity in abdominal ganglia of the migratory locust. Cell Tissue Res 267:85–98
Giebultowicz JM, Truman JW (1984) Sexual differentiation in the terminal ganglion of the moth Manduca sexta: role of sex-specific cell death. J Comp Neurol 226:87–95
Goodman CS (1982) Embryonic development of identified neurones in the grasshopper. In: Spitzer NC (ed) Neuronal development. Plenum Press, New York, pp 171–212
Heckmann R (1988) Die Funktion der Innervierung der Genital-kammer von Acheta domesticus (L). Diplomarbeit, Universität Konstanz
Kalogianni E, Pflüger H-J (1992) The identification of motor and unpaired median neurones innervating the locust oviduct. J Exp Biol 168:177–198
Kimura T, Yasuyama K, Yamaguchi T (1989) Proctolinergic innervation of the accessory glands in male crickets (Gryllus bimaculatus): detection of proctolin and some pharmacological properties of myogenically and neurogenically evoked contractions. J Insect Physiol 35:251–264
Kiss T, Varanka I, Benedeczky I (1984) Neuromuscular transmission in the visceral muscle of locust oviduct. Neuroscience 12:309–322
Klemm N (1976) Histochemistry of putative transmitter substances in the insect brain. Prog Neurobiol 7:99–169
Konings PNM, Vullings HGB, Geffard M, Buijs RM, Diederen JHB, Jansen WF (1988) Immunocytochemical demonstration of octopamine-immunoreactive cells in the nervous system of Locusta migratoria and Schistocerca gregaria. Cell Tissue Res 251:371–379
Lange AB, Orchad I (1984) Dorsal unpaired median neurons and ventral bilaterally paired neurones, project to a visceral muscle in an insect. J Neurobiol 15:441–453
Lange AB, Orchard I (1986) Ventral neurons in an abdominal ganglion of the locust Locusta migratoria, with properties similar to dorsal unpaired median neurons. Can J Zool 64:264–267
Nürnberger A, Rapus J, Eckert M, Penzlin H (1990) Taurine-, octopamine-, and proctolin-like immunoreactivity in neurons of the American cockroach Periplaneta americana L. In: Elsner N, Roth G (eds) Brain — perception cognition. Proceedings of the 18th Göttingen Neurobiology Conference. Thieme, Stuttgart, p 490
O'Shea M, Adams ME (1981) Pentapeptide (proctolin) associated with an identified neuron. Science 213:567–569
Orchard I, Lange AB (1985) Evidence for octopaminergic modulation of an insect visceral muscle. J Neurobiol 16:171–181
Orchard I, Lange AB, Cook H, Ramirez J-M (1989) A subpopulation of dorsal unpaired median neurons in the blood-feeding insect Rhodnius prolixus displays serotonin-like immunoreactivity. J Comp Neurol 289:118–128
Pflüger H-J, Watson ADH (1988) Structure and distribution of dorsal unpaired median (DUM) neurones in the abdominal nerve cord of male and female locusts. J Comp Neurol 268:329–345
Pflüger H-J, Witten JL, Levine RB (1993) Fate of abdominal ventral unpaired median (VUM) neurones during metamorphosis of the hawkmoth, Manduca sexta. J Comp Neurol (in press)
Roonwal ML (1937) Studies on the embryology of the African migratory locust, Locusta migratoria migratorioides Reiche and Frm. Philos Trans R Soc [Biol] 227:175–244
Seabrook WD (1968) The innervation of the terminal abdominal segments (VIII–XI) of the desert locust, Schistocerca gregaria. Canadian Entomologist 100:693–715
Spörhase-Eichmann U, Vullings HGB, Buijs RM, Hörner M (1992) Octopamine-immunoreactive neurones in the central nervous system of the cricket Gryllus bimaculatus. Cell Tissue Res 268:287–304
Stevenson PA, Pflüger H-J, Eckert M, Rapus J (1992a) Octopamine immunoreactive cell population in locust thoracic-abdominal nervous system. J Comp Neurol 315:382–397
Stevenson PA, Ferber M, Pflüger H-J (1992b) Co-localisation of octopamine- & FMRFamide/BPP-like immunoreactivity in an identified locust DUM-neurone. In: Elsner N, Richter DW (eds) Rhythmogenesis in neurones and networks. Proceedings of the 20th Göttingen Neurobiology Conference. Thieme, Stuttgart New York p 479
Stoya GM, Agricola H, Eckert M, Penzlin H (1989) Investigations on the innervation of the oviduct muscle of the cockroach, Periplaneta americana (L). Zool Jb Physiol 93:75–86
Thompson KJ (1986) Oviposition digging in the grasshopper 1. Functional anatomy and the motor programme. J Exp Biol 122:387–411
Thompson KJ, Siegler MVS (1989) Properties of the small dorsal unpaired median (DUM) neurons of the grasshopper. Soc Neurosci Abstr 15:1296
Thompson KJ, Siegler MVS (1991) Anatomy and physiology of spiking local and intersegmental interneurons in the median neuroblast lineage of the grasshopper. J Comp Neurol 305:659–675
Thorn RS, Truman JW (1989) Sex-specific neuronal respecification during the metamorphosis of the tobacco hornworm moth Manduca sexta. J Comp Neurol 284:489–503
Tyrer NM, Gregory GE (1982) A guide to the neuroanatomy of locust suboesophageal and thoracic ganglia. Philos Trans R Soc Lond [Biol] 297:91–123
Watson ADH (1984) The dorsal unpaired median neurons of the locust metathoracic ganglion: neuronal structure and diversity, and synaptic distribution. J Neurocytol 13:303–327
Whim MD, Evans PD (1989) Age-dependence of octopaminergic modulation of flight muscle in the locust. J Comp Physiol [A] 165:125–137
Yamaguchi T, Kushiro N, Waki T (1985) Sexual dimorphism of the terminal abdominal ganglion of the cricket. Naturwis-senschaften 72:153–154
Yasuyama K, Kumura T, Yamaguchi T (1988) Musculature and innervation of the internal reproductive organs in the male cricket, with special reference to the projection of unpaired median neurones of the terminal abdominal ganglion. Zool Sci 5:767–780
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Stevenson, P.A., Pflüger, HJ., Eckert, M. et al. Octopamine-like immunoreactive neurones in locust genital abdominal ganglia. Cell Tissue Res 275, 299–308 (1994). https://doi.org/10.1007/BF00319428
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DOI: https://doi.org/10.1007/BF00319428