Summary
The Fo-ATPase subunit 6 gene (atp6) of rapeseed mitochondria has been isolated from both pol malesterile and normal (fertile) cytoplasms in order to determine whether the rearrangements around the atp6 locus in pol male-sterile cytoplasm play a role in cytoplasmic male-sterility (cms). The pol cms and normal atp6 genes are identical and encode a 261-amino acid polypeptide. As a result of extensive rearrangement, a novel reading frame (pol-urf) was generated upstream of the atp6 gene only in pol cms mitochondria, which encoded 105 amino acids and might be co-transcribed with atp6. A 5′-portion of pol-urf shows sequence homology to the Oenothera ORFB gene associated with coxIII. A 5′-flanking region of the pol-urf also shows homology to that of ORF105 in Ogura cms radish mitochondria. These DNA rearrangements which give rise to pol-urf in the vicinity of the atp6 locus may be responsible for cms in rapeseed.
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Communicated by C. W. Birky, Jr.
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Handa, H., Nakajima, K. Different organization and altered transcription of the mitochondrial atp6 gene in the male-sterile cytoplasm of rapeseed (Brassica napus L.). Curr Genet 21, 153–159 (1992). https://doi.org/10.1007/BF00318475
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DOI: https://doi.org/10.1007/BF00318475