Summary
We cloned all of Adiantum capillus-veneris chloroplast DNA PstI fragments longer than 1.0 kb, which cover 98% of the genome. These cloned fragments were used to construct a physical map for five restriction enzymes. The genome of A. capillus-veneris is approximately 153 kb long and contains a 24 kb inverted repeat. Mapping of 12 chloroplast DNA genes and heterologous hybridization, involving A. capillus-veneris chloroplast DNA and angiosperm chloroplast DNA probes, demonstrated that chloroplast DNA of A. capillus-veneris has a different gene order from typical angiosperm cpDNA (e.g., tobacco) in the inverted repeat region and the flanking segment of the large single copy region.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Birky CW Jr (1988) Evolution and variation in plant chloroplast and mitochondrial genomes. In: Gottlieb LD, Jain SK (eds) Evolutionary biology. Chapman and Hall, London New York, pp 23–53
Bovenberg WA, Howe CH, Kool AJ, Kijkamp HJJ (1984a) Curr Genet 8:283–290
Bovenberg WA, Howe CH, Kool AJ, Kijkamp HJJ (1984b) Curr Genet 8:231–241
Calie PJ, Hughes KW (1987) Mol Gen Genet 208:335–341
Chu NM, Tewari KK (1982) Mol Gen Genet 186:23–32
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Feinberg AP, Vogelstein B (1984) Addendum Anal Biochem 137:266–267
Hirai A, Ishibashi T, Morikami A, Iwatsuki N, Shinozaki K, Sugiura M (1985) Theor Appl Genet 70:117–122
Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun CR, Meng BY, Li YQ, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M (1989) Mol Gen Genet 217:185–194
Jansen RK, Palmer JD (1987) Curr Genet 11:553–564
Koller B, Delius H (1980) Mol Gen Genet 178:261–269
Kolodner R, Tewari KK (1979) Proc Natl Acad Sci USA 76:41–45
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor New York
Ohyama K, Fukuzawa H, Kohch T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, chang Z, Aota SI, Inokuchi H, Ozeki H (1986) Nature 322:572–574
Palmer JD (1985a) Annu Rev Genet 19:325–354
Palmer JD (1985b) Evolution of chloroplast and mitochondrial DNA in plants and algae. In: MacIntyre RJ (eds) Molecular evolutionary biology. Plenum, New York, pp 131–240
Palmer JD (1986) Methods Enzymol 118:167–186
Palmer JD, Stein DB (1982) Curr Genet 5:165–170
Palmer JD, Stein DB (1986) Curr Genet 10:823–833
Palmer JD, Thompson WF (1981) Proc Natl Acad Sci USA 78:5533–5537
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Theor Appl Genet 65:181–189
Palmer JD, Nugent JM, Herbon LA (1987) Proc Natl Acad Sci USA 84:769–773
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Smith GE, Summers MD (1980) Anal Biochem 109:123–129
Sugiura M, Shinozaki K, Zaita N, Kusuda M, Kumano M (1986) Plant Science 44:211–216
Yanisch-Perron C, Vieira J, Messing J (1985) Gene 33:103–119
Zhou DX, Massenet O, Quingley F, Marison MJ, Moneger F, Huber P, Mache R (1988) Curr Genet 13:433–439
Author information
Authors and Affiliations
Additional information
Communicated by B. B. Sears
Rights and permissions
About this article
Cite this article
Hasebe, M., Iwatsuki, K. Chloroplast DNA from Adiantum capillus-veneris L., a fern species (Adiantaceae); clone bank, physical map and unusual gene localization in comparison with angiosperm chloroplast DNA. Curr Genet 17, 359–364 (1990). https://doi.org/10.1007/BF00314885
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00314885