Summary
A set of probes that detect restriction fragment length polymorphisms (RFLPs) in nuclear DNA has been developed for genetic studies of the phytopathogenic fungus Septoria tritici. Two plasmid libraries containing 0.5–1.3 or 1.3–2.4 kb fragments of S. tritici nuclear DNA were constructed. Seventeen random clones from each library were used as probes to screen for RFLP variation among a geographically-diverse group of six S. tritici isolates. Among the 196 probe-enzyme combinations tested, 145 detected RFLPs among the six isolates. The restriction enzymes EcoRV and PstI detected RFLPs most efficiently. Three probes detected deletions. A ribosomal DNA probe from yeast did not detect a significant amount of variation. These probes will be useful for studying genetic variation, population genetics, and genome organization of S. tritici.
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Communicated by B. B. Sears
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McDonald, B.A., Martinez, J.P. Restriction fragment length polymorphisms in Septoria tritici occur at a high frequency. Curr Genet 17, 133–138 (1990). https://doi.org/10.1007/BF00312858
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DOI: https://doi.org/10.1007/BF00312858