Abstract
A simple method, using silver impregnation after 2 x SSC pretreatment allowed us to demonstrate axial structures (cores) in the metaphase I bivalents of the neo-XY race of Pycnogaster cucullata under bright-field microscopy. Axial structures can also be shown in DNA-depleted metaphase I bivalents, suggesting that DNA is not essential to demonstrate these elements. Specifically differentiated regions, which coincide with kinetochores, secondary constrictions (including the NOR), and chiasmata were also found. These regions have a characteristic morphology and therefore may be utilized for cytogenetic analysis. The simultaneous visualization of all these regions allowed us to establish their spatial relationships and hence the basic structural organization of the neo-XY sex bivalent in this species.
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Sentís, C., Rodriguez-Campos, A., Stockert, J.C. et al. Morphology of the axial structures in the neo-XY sex bivalent of Pycnogaster cucullata (Orthoptera) by silver impregnation. Chromosoma 90, 317–321 (1984). https://doi.org/10.1007/BF00287041
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DOI: https://doi.org/10.1007/BF00287041