Summary
Plants were obtained from protoplasts of shoot cultures of potato (Solarium tuberosum L. cv. ‘Maris Bard’) and from in situ calluses upon plants of cv. ‘Majestic’. None of the protoplast-derived plants resembled each other in all of ten morphological characteristics scored and only one resembled the parental ‘Maris Bard’ type. As there were a number of plants regenerated from each of ten protoplast-derived calluses it is concluded that variation arose after protoplast isolation during the cell culture phase. Plants regenerated from in situ calluses of cv. ‘Majestic’ were quite uniform. Reported cases of variation and uniformity from cultured potato tissues are discussed. It is concluded that the variation is not a consequence of using protoplasts and that the expression or induction of variation is controllable.
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Literature
Ahloowalia, B.S. (1978) Novel ryegrass genotypes regenerated from embryo-callus culture. In: Frontiers of Plant Tissue Culture (ed. Thorpe, T.A.), p. 515. Calgary, Canada: University of Calgary
Bayliss, M.W. (1980) Chromosomal variation in plant tissues in culture. Intern. Rev. Cytol. Suppl. 11A, 113–144
Harten, A.M., van; Boulter, H.; Broertjes, C. (1981) In vitro adventitious bud techniques for vegetative propagation and mutation breeding of potato (Solanum tuberosum L.) II: Significance for mutation breeding. Euphytica 30, 1–8
Heinz, D.J.; Krishnamurthi, M.; Nickell, L.G.; Maretzki, A. (1977) Cell, tissue and organ culture in sugar cane improvement. In: Plant Cell Tissue and Organ Culture (eds: Reinert, J; Bajaj, Y.P.S.), pp. 3–17. Berlin, Heidelberg, New York: Springer
Larkins, P.J.; Scowcroft, W.R. (1981) Somaclonal variation — a novel source of variability from cell cultures. Theor. Appl. Genet. 60, 197–214
Murashige, T.; Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497
National Institute of Agricultural Botany (1975): Guide to the Identification of Potato Varieties, Cambridge, UK
Shepard, J.F.; Bidney, D.; Shahin, E. (1980) Potato protoplasts in crop improvement. Science 208, 17–24
Thomas, E. (1981) Plant regeneration from shoot culturederived protoplasts of tetraploid potato (Solanum tuberosum cv. ‘Maris Bard’). Plant Sci. Lett. 23, 84–88
Wenzel, G.; Schieder, O.; Przewozny, T.; Sopory, S.K.; Melchers, G. (1979) Comparison of single cell culture-derived Solanum tuberosum L. plants and a model for their application in breeding programs. Theor. Appl. Genet. 55, 49–55
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Communicated by P. Maliga
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Thomas, E., Bright, S.W.J., Franklin, J. et al. Variation amongst protoplast-derived potato plants (Solatium tuberosum cv. ‘Maris Bard’). Theoret. Appl. Genetics 62, 65–68 (1982). https://doi.org/10.1007/BF00276285
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DOI: https://doi.org/10.1007/BF00276285