Abstract
Plant regeneration via somatic embryogenesis was obtained from pea protoplasts. Strong auxins (picloram or 2.4-D) and increased osmolarity of the medium were necessary for embryo induction. Relatively high amounts of embryogenic calli could be obtained in 2 genotypes. After a period on hormone-free medium, a second induction of somatic embryos was possible. Further development of somatic embryos was accomplished on GA3 — containing medium.
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Abbreviations
- ABA:
-
abscisic acid
- BA:
-
6-benzylaminopurine
- 2.4-D:
-
2.4-dichlorophenoxyacetic acid
- GA3 :
-
gibberellic acid
- Kin:
-
kinetin
- NAA:
-
naphthaleneacetic acid
- Pic:
-
Picloram, 4-amino-3,5,6-trichloropicolinic acid
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Communicated by I. Potrykus
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Lehminger-Mertens, R., Jacobsen, HJ. Plant regeneration from pea protoplasts via somatic embyogenesis. Plant Cell Reports 8, 379–382 (1989). https://doi.org/10.1007/BF00270073
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DOI: https://doi.org/10.1007/BF00270073