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Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.)

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Summary

Seventeen cultivars of cotton (Gossypium hirsutum L.) were evaluated for callus initiation and maintenance using 3 initiation media and 3 maintenance media. After a series of transfers of a 3% glucose media, calli were placed on a 3% sucrose medium. After several weeks calli were observed for the presence of embryo-like structures. Cultivars Coker 201 and Coker 315 were identified as embryogenic. Embryogenic callus has since been routinely obtained within 6 weeks by initiating callus on glucose media for 3–4 weeks followed by transfer to sucrose media. Histological examination has shown that embryos are derived from isodiametric, densely cytoplasmic cells and follow predictable patterns of development. Upon maturity, transfer to auxin-free media with reduced sucrose levels results in embryo germination. Regenerated plants can be transferred to greenhouse within 90 days of callus initiation.

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Author notes

  1. R. C. Shoemaker

    Present address: Department of Genetics and Agronomy, Iowa State University, 50011, Ames, IA, USA

Authors and Affiliations

  1. School of Biological Sciences, University of Nebraska-Lincoln, 348 Manter Hall of Life Sciences, 68588-0118, Lincoln, NE, USA

    R. C. Shoemaker, L. J. Couche & D. W. Galbraith

Authors
  1. R. C. Shoemaker
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  2. L. J. Couche
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  3. D. W. Galbraith
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Additional information

Communicated by P. Maliga

The senior author is presently a Research Geneticist, USDA-ARS, and Assistant Professor Present address

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Shoemaker, R.C., Couche, L.J. & Galbraith, D.W. Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Reports 5, 178–181 (1986). https://doi.org/10.1007/BF00269112

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  • DOI: https://doi.org/10.1007/BF00269112

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