Summary
A special class of transducing particles in lysates of P22 is described. When DNA synthesized after infection is density labelled with bromodeoxyuridine, this class of particles exhibit a higher density in CsCl-gradients than normal transducing particles in the same lysates. It is shown that the DNA of these transducing particles carries the normal amount of bacterial information and is synthesized in the semiconservative mode after phage-infection. From the efficiency of BU-incorporation we conclude that the DNA molecules of these particles are not products of normal bacterial replication, but might be replicated under the control of the phage.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beyersmann, D., Schuster, H.: DNA synthesis in P1 infected E. coli mutants temperature-sensitive in DNA replication. Molec. gen. Genet. 114, 173–176 (1972)
Chan, H., Lark, K. G.: Chromosome replication in Salmonella typhimurium. J. Bact. 97, 848–860 (1969)
Dopatka, M. D., Prell, H. H.: Amber mutants of Salmonella-phage P22 in genes engaged in the establishment of lysogeny. Molec. gen. Genet. 120, 157–170 (1973)
Ebel-Tsipis, J., Botstein, D., Fox, M. S.: Generalized transduction by bacteriophage P22 in Salmonella typhimurium. I. Molecular origin of transducing DNA. J. molec. Biol. 71, 433–448 (1972)
Eberle, H.: A comparison of 14C-glucose 15NH4Cl with 5-bromouracil as density label in two experiments on sequential DNA replication in Escherichia coli 15 T. J. molec. Biol. 31, 149–152 (1968)
Lin, S. Y., Riggs, A. D.: Lac-operator analogues: bromodeoxyuridine substitution in the lac-operator affects the rate of dissociation of the lac-repressor. Proc. nat. Acad. Sci. (Wash.) 69, 2574–2576 (1972)
Roth, J. R., Hartman, P. E.: Heterogeneity in P22 transducing particles. Virology 27, 297–307 (1965)
Schmieger, H.: Die molekulare Struktur transduzierender Partikel beim Salmonella-Phagen P22. I. Dichtegradientenuntersuchungen an intakten Phagen. Molec. gen. Genet. 102, 336–347 (1968)
Schmieger, H.: The molecular structure of the transducing particles of Salmonella phage P22. II. Density gradient analysis of DNA. Molec. gen. Genet. 109, 323–337 (1970)
Schmieger, H.: A method for detection of phage mutants with altered transducing ability. Molec. gen. Genet. 110, 378–381 (1971)
Schmieger, H.: Phage P22-mutants with increased or decreased transduction abilities. Molec. gen. Genet. 119, 75–88 (1972)
Schmieger, H., Backhaus, H.: The origin of DNA in transducing particles in P22-mutants with increased transduction-frequencies (HT-mutants). Molec. gen. Genet. 120, 181–190 (1973)
Sheppard, D. E.: Density gradient centrifugation of bacteriophage P22. Virology 17, 212–214 (1962)
Smith, H. O., Levine, M.: The synthesis of phage and host DNA in the establishment of lysogeny. Virology 25, 585–590 (1965)
Author information
Authors and Affiliations
Additional information
Communicated by G. Bertani
Rights and permissions
About this article
Cite this article
Backhaus, H., Schmieger, H. The origin of the DNA in a special class of generalized transducing particles of Salmonella-phage P22 . Molec. Gen. Genet. 131, 123–135 (1974). https://doi.org/10.1007/BF00266148
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00266148