Summary
Hybridization of synthetic allotetraploids of S. pinnatisectum with S. etuberosum (4x-EP) with S. acaule (2n = 4x = 48) resulted in two individuals that were highly fertile, in contrast to all other progenies. The unique individuals are hexaploids, 2n = 72, while the other progenies are tetraploids, 2n = 48. They are thought to be the products of a union between 2n eggs of S. acaule and normal 1n microspores of 4x-EP. The fertile hexaploids (designated 6x-AEP) produced abundant selfed seed and viable hybrids with cultivated diploid potato, S. phureja, when developing embryos were rescued from berries and cultured before transplanting to pot culture. The extreme variability in chromosome constitution of the hybrids with S. phureja and selfed progenies indicates that addition and substitution lines of etb chromosomes bearing genes of interest to breeders could easily be produced from this material. The production of sesquiploids, as the 6x-AEP hybrids are called, is discussed as a useful bridging step in the introduction of alien genes from genomes that share little homology with the cultivated genome.
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References
Brown CR (1980) Incorporation of virus resistance and future plans. In Report Plan Conf Strategy Virus Manage Potatoes. CIP, Lima, Peru
Chavez R, Brown CR, Iwanaga M (1988) Transfer of resistance to PLRV titer buildup from Solanum etuberosum to a tuberbearing Solanum gene pool. Theor Appl Genet 76:129–135
DeVerna JW, Chetelat RT, Rick CM, Stevens MA (1987) Introgression of Solanum lycopersicoides germplasm. In: Nevins DJ, Jones RA (eds) Tomato biotechnology. Liss, New York, 339 pp
Goodspeed TH, Bradley MV (1942) Amphidiploidy. Bot Rev 8:271–316
Hermsen JGTh (1980) Recent progress and future plans for utilizing Mexican wild species for pest and disease resistance. In: Report Plan Conf Util Genet Resourcs Potato III. CIP, Lima, Peru
Hermsen JGTh, Taylor LM (1979) Successful hybridization of non-tuberous Solanum etuberosum Lindl. and tuber-bearing S. pinnatisectum Dun. Euphytica 28:1–7
Hermsen JGTh, Ramanna MS, Sawor Z (1981) The effect of chromosome doubling on fertility, meiotic behavior and crossability of S. etuberosum x S. pinnatisectum. Euphytica 30:33–39
Jones RAC (1979) Resistance to potato leaf roll in Solanum brevidens. Potato Res 22:149–152
Marks GE (1954) An aceto carmin glycerol jelly for use in pollen fertility counts. Stain Technol 29:277
Mortenson LR, Peloquin SJ, Hougas RW (1964) Germination of Solanum pollen of artificial media. Am Potato J 41:322–328
Ramanna MS, Hermsen JGTh (1981) Structural hybridity in the series Etuberosa of the genus Solanum and its bearing on crossability. Euphytica 30:15–31
Rick CM, DeVerna JW, Chetelat RT, Stevens MA (1986) Meiosis in sesquidiploid hybrids of Lycopersicon esculentum and Solanum lycopersicoides. Proc Natl Acad Sci USA 83:3580–3583
Wangenheim, K.-H. von 1954. Zur Ursache der Kreuzungsschwierigkeiten zwischen Solanum tuberosum L. und S. acaule Bitt. bzw. S. stoloniferum Schlechtd. et Bouche. Z Pflanzenzücht 34:7–48
Webber JM (1930) Interspecific hybridization in Nicotiana. XI. The cytology of a sesquidiploid hybrid between tabacum and sylvestris. Univ Calif Publ Bot 11:319–354
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Communicated by G. Wenzel
This work was part of the requirement for a Ph.D. degree of the senior author R. Chavez at The University of Birmingham, UK
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Chavez, R., Brown, C.R. & Iwanaga, M. Application of interspecific sesquiploidy to introgression of PLRV resistance from non-tuber-bearing Solanum etuberosum to cultivated potato germplasm. Theoret. Appl. Genetics 76, 497–500 (1988). https://doi.org/10.1007/BF00260898
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DOI: https://doi.org/10.1007/BF00260898