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A simple, versatile feeder layer system for Brassica oleracea protoplast culture

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Abstract

Protoplasts from cauliflower (Brassica oleracea ssp. botrytis) and broccoli (ssp. italica) leaves and hypocotyls were successfully cultured on membrane filters over a feeder layer of cells from a B. campestris suspension culture. Cells from rice, tomato and tobacco suspensions were not as effective as the B. campestris cells. Plants were recovered from protoplasts of previously recalcitrant Brassica genotypes. Protoplasts cultured in low numbers (10–100) on the feeder layer divided and formed colonies capable of plant regeneration, as did fused protoplasts.

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Abbreviations

BA:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

indole-3-acetic acid

NAA:

naphthaleneacetic acid

PCV:

packed cell volume

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Authors and Affiliations

  1. Department of Plant Breeding and Biometry, Cornell University, 14853-1902, Ithaca, NY, USA

    Thomas W. Walters & Elizabeth D. Earle

Authors
  1. Thomas W. Walters
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  2. Elizabeth D. Earle
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Communicated by J. M. Widholm

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Walters, T.W., Earle, E.D. A simple, versatile feeder layer system for Brassica oleracea protoplast culture. Plant Cell Reports 9, 316–319 (1990). https://doi.org/10.1007/BF00232859

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  • DOI: https://doi.org/10.1007/BF00232859

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