Abstract
Adventitious shoots were obtained from leaf and stem callus of Eucalyptus tereticornis SM. Callus was induced on B5 medium with 0.1 mg/l benzyladenine (BA) and 3 or 5 mg/l naphthalene acetic acid in the dark. Shoot initiation occurred on modified Woody Plant medium (mWP) containing 0.5 mg/l BA, 500 mg/l polyvinylpyrrolidone and 10% (v/v) coconut milk. Multiple shoots were also regenerated directly from hypocotyl segments of 4 to 6 week old seedlings on B5 medium with 0.5 mg/l BA. Regenerated shoots could be rooted with 100% efficiency on mWP medium containing 0.5 mg/l indolebutyric acid and transferred to soil in the greenhouse. Suspension cultures were obtained from the callus using B5 medium with 0.5 mg/l 2,4-dichlorophenoxyacetic acid. Callus clumps grew from less than 1 mm to 4–6 mm in diameter within two weeks on transfer to shoot regeneration medium but failed to form shoots or somatic embryos.
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Abbreviations
- BA:
-
Benzyladenine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IBA:
-
Indolebutyric acid
- NAA:
-
Naphthaleneacetic acid
- PVP:
-
Polyvinylpyrrolidone
- mWP:
-
modified Woody Plant medium
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Communicated by A. R. Gould
Scientific Contribution No. 1689 from New Hampshire Agricultural Experiment Station.
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Subbaiah, M.M., Minocha, S.C. Shoot regeneration from stem and leaf callus of Eucalyptus tereticornis . Plant Cell Reports 9, 370–373 (1990). https://doi.org/10.1007/BF00232401
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DOI: https://doi.org/10.1007/BF00232401