Summary
The plant regeneration ability of callus obtained from zygotic embryos of the monocot Alstroemeria spp. was studied. The best explants for somatic embryogenesis were immature zygotic embryos in half-ovules when the endosperm was still soft and white. For 2 genotypes embryogenic callus was induced on callus induction medium with a success rate of 54%. The best callus induction period was 10 weeks. The morphology of embryogenic callus was nodular. Somatic embryos were formed after transfer of the callus to regeneration medium. These somatic embryos revealed later on the typical features of zygotic Alstroemeria embryos. The total duration of the plant regeneration protocol, from inoculation till rooted plantlets ready for transfer to the greenhouse, was 28 weeks.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BAP:
-
6-benzylaminopurine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- MS:
-
Murashige and Skoog (1962)
- NAA:
-
α-naphthaleneacetic acid
References
Bennini A, Caffaro L, Dameri RM, Gastaldo P, Profumo P (1988) Euphytica 39:255–263.
Bhaskaran S, Smith RH (1990) Crop Science 3:1328–1336.
Bridgen MP, Langhans R, Graig R (1989) Herbertia 45:93–96.
Buitendijk JH, Ramanna MS, Jacobsen E (1992) Acta Hort 325:493–498.
Christou P (1992) Plant J 2:275–281.
Esau K (1977) Anatomy of seed plants. Wiley, New York pp 491–499.
Goldstein CS, Kronstad WE (1986) Theor Appl Genet 71:631–636.
Gonzalez-Benito ME (1990) PhD Thesis, University of Nottingham.
Gonzalez-Benito ME, Alderson PG (1992) J Exp Botany 43:205–211.
Hutchinson MJ, Tsujita JM, Saxena PK (1994) Plant Cell Reports 14:184–187.
Lörz H, Göbel E, Brown P (1988) Plant Breeding 100:1–25.
Monette PL (1992) In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, vol 20. High-Tech and Microprop IV. Springer, Berlin pp 1–18.
Murashige T, Skoog F (1962) Physiol Plant 15:473–497.
Pierik RLM, van Voorst A, Booy G, van Acker CAM, Lelivelt CLC, de Wit JC (1988) Acta Hort 226:81–89.
Potrykus I (1991) Plant Mol Biol 42:205–225.
Raemakers CJJM, Jacobsen E, Visser RGF (1995) Euphytica 81:93–107.
Rotem-Abarbanell D, Breiman A (1989) Plant Cell Tissue and Organ Culture 16:207–216.
Rueb S, Leneman M, Schilperoort RA, Hensgens LAM (1994) Plant Cell, Tissue and Organ Culture 36:259–264.
Teixeira JB, Söndahl MR, Kirby EG (1993) Plant Cell, Tissue and Organ Culture 34:227–233.
Vasil IK (1987) J Plant Physiol 128:193–218.
Ziv M, Kanterovitz R, Halevy AH (1973) Sci Hortic 1:271–277.
Author information
Authors and Affiliations
Additional information
Communicated by H. Lörz
Rights and permissions
About this article
Cite this article
Van Schaik, C.E., Posthuma, A., De Jeu, M.J. et al. Plant regeneration through somatic embryogenesis from callus induced on immature embryos of Alstroemeria spp. L.. Plant Cell Reports 15, 377–380 (1996). https://doi.org/10.1007/BF00232375
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232375