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Somatic embryogenesis and regeneration of flowering plants in rose

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Summary

Somatic embryos of the cut rose cultivars ‘Domingo’ and ‘Vickey Brown’ were obtained from callus derived from leaf explants on half strength Murashige and Skoog medium with low concentrations of kinetin and 1-naphthyl acetic acid or 2-naphthyloxyacetic acid. Somatic embryos were first observed after 6 to 12 weeks of culture on callus formed at the basis or midrib of the leaf. Embryos could be grown to phenotypically true to type greenhouse plants.

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Abbreviations

IAA:

3-indole-acetic acid

IBA:

indole-3-butyric acid

NAA:

1-naphthylacetic acid

NOA:

2-naphthyloxyacetic acid

BAP:

6-benzylaminopurine

KIN:

6-furfurylaminopurine

MS:

Murashige and Skoog

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Authors and Affiliations

  1. Terra Nigra B. V., Iepenlaan 48, 1424, PA De Kwakel, Holland

    Joyce C. de Wit & Harald F. Esendam

  2. Kemira Oy, Espoo Research Centre, P.O. Box 44, SF-02271, Espoo, Finland

    Jarmo J. Honkanen & Ulla Tuominen

Authors
  1. Joyce C. de Wit
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  2. Harald F. Esendam
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  3. Jarmo J. Honkanen
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  4. Ulla Tuominen
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Communicated by H. Lörz

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de Wit, J.C., Esendam, H.F., Honkanen, J.J. et al. Somatic embryogenesis and regeneration of flowering plants in rose. Plant Cell Reports 9, 456–458 (1990). https://doi.org/10.1007/BF00232272

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  • DOI: https://doi.org/10.1007/BF00232272

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