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Sequence and evolution of cattle MHC class I cDNAs: concerted evolution has not taken place in cattle

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Abstract

To explore genetic mechanisms responsible for major histocompatibility complex (MHC) class I evolution in the artiodactyls, we cloned and sequenced MHC class I cDNAs from a Bos taurus bull heterozygous for cattle MHC (BoLA) class I serological specificities w2 and w30. Four unique cDNAs were found, indicating the presence of at least two MHC class I loci. Analysis of these four cDNAs and all previously published BoLA cDNA sequences suggested that there may be three cattle MHC class I loci. Additionally, comparison of all of the BoLA class I cDNAs to MHC class I cDNAs of other artiodactyls showed that some of the BoLA class I cDNAs were more similar to certain sheep cDNAs than they were to other cattle cDNAs. These data indicate that each BoLA class I locus has evolved independently after an ancestral gene duplication event and that inter-locus segmental exchange o or concerted evolution has not occurred rapidly enough to cause extensive divergence between the orthologous MHC class I loci of sheep and cattle.

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  1. Theodore L. Garber

    Present address: Wisconsin Regional Primate Research Center, University of Wisconsin, 1220 Capitol Court, 53715-1299, Madison, WI, USA

Authors and Affiliations

  1. Department of Veterinary Pathobiology, Texas A & M University, 77843, College Station, TX, USA

    Theodore L. Garber & Joe W. Templeton

  2. Department of Biology, Pennsylvania State University, 16802, University Park, PA, USA

    Austin L. Hughes

  3. New England Regional Primate Research Center, Harvard Medical School, 01772, Southborough, MA, USA

    Norman L. Letvin

  4. Dept. of Pathology and the Wisconsin Regional Primate Research Center, University of Wisconsin, 1220 Capitol Court, 53715-1299, Madison, WI, USA

    David I. Watkins

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  1. Theodore L. Garber
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Additional information

The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers L02832–L02835.

Correspondence to: T. L. Garber at the present address.

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Garber, T.L., Hughes, A.L., Letvin, N.L. et al. Sequence and evolution of cattle MHC class I cDNAs: concerted evolution has not taken place in cattle. Immunogenetics 38, 11–20 (1993). https://doi.org/10.1007/BF00216385

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  • DOI: https://doi.org/10.1007/BF00216385

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