Summary
The localization of A and B antigens in the organs of blood group AB individuals has been studied using a double immunoenzymatic labeling method. Both A and B antigens were found in the various epithelial cells of these organs, but the epithelial cells could be classified into the following four types depending on the reaction pattern with anti-A and anti-B sera: type 1: cells that stained positive with both anti-A and anti-B sera; type 2: cells that stained positive with anti-A serum only; type 3: cells that stained positive with anti-B serum only; type 4: cells that were negative with both sera. The distribution of each of these epithelial cell types varied considerably, even in the same tissue and individual. Our results seem to suggest that a dissociation in the conversion to the A and B antigens occurs in the tissue of individuals belonging to blood group AB and that the degree of this dissociation varies from tissue to tissue and from cell to cell.
Zusammenfassung
Die Lokalisation der A- und B-Antigene in den Organen von Personen der Blutgruppe AB wurde mit einer doppel-immunenzymatischen Methode studiert. In den verschiedenen Epithelzellen dieser Organe konnten beide Antigene nachgewiesen werden. Im Hinblick auf die A- und B-Antigene konnten 4 verschiedene Reaktionsmuster klassifiziert werden: Typ 1: Zellen, welche sich sowohl mit Anti-A als auch mit Anti-B-Seren anfärben ließen; Typ 2: Zellen, welche sich nur mit Anti-A-Serum anfdrben ließen; Typ 3: Zellen, welche sich nur mit Anti-B-Serum anfdrben ließen; Typ 4: Zellen, welche mit beiden Seren negativ reagierten. Die Verteilung jedes dieser epithelialen Zelltypen variierte betrachtlich, auch innerhalb desselben Gewebes und derselben Person. Unsere Ergebnisse legen die Vermutung nahe, daß bei Personen der Blutgruppe AB in der Phase der Konversion zu den A- and B-Antigenen eine Dissoziation stattfindet and daß das Ausmaß zu dieser Dissoziation von Gewebe zu Gewebe and von Zelle zu Zelle variiert.
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Bunai, Y., Nagai, A. & Ohya, I. Distribution of A and B antigens in organs of blood group AB individuals: observations disclosed by a double immunoenzymatic labeling method. Z Rechtsmed 103, 557–565 (1990). https://doi.org/10.1007/BF00202715
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DOI: https://doi.org/10.1007/BF00202715