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Morphogenesis of potato plants in vitro. I. Effect of light quality and hormones

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Abstract

Stem cuttings of potato plants (Solanum tuberosum L., cv. Miranda) were cultured in vitro on MS medium with sucrose either without or with addition of indole-3-acetic acid (IAA) or kinetin (K) under red light (R) or blue light (B). Plants on medium without hormones under R were thin, long, with very small leaves, and produced no or only a few microtubers (after longer-lasting cultivations). In B, plants remained short, thick, with large, wellde-veloped leaves and produced a significant amount of microtubers. Darkening of both roots and shoots strongly promoted tuber formation; the tubers were formed on the darkened part of the plant. IAA had no pronounced effect on plant development in B except for slight lengthening of the stem, and, in longer cultivations, slightly enhanced tuber formation as well. In R, IAA brought about several significant effects: stem reduction and induction of tuber formation being the most significant. Kinetin in R increased tuber formation slightly. In B, kinetin not only strongly stimulated tuber formation, but also increased the total fresh weight and root (+ stolons)/shoot ratio. Results are discussed with regard to the possible role of auxins and/or cytokinins in mediating the morphogenetic effects of light.

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Authors and Affiliations

  1. Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia

    Nina P. Aksenova, Tatyana N. Konstantinova, Lydiya I. Sergeeva & Svetlana A. Golyanovskaya

  2. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha, Czech Republic

    Ivana Macháčková

Authors
  1. Nina P. Aksenova
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  2. Tatyana N. Konstantinova
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  3. Lydiya I. Sergeeva
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  4. Ivana Macháčková
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  5. Svetlana A. Golyanovskaya
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Aksenova, N.P., Konstantinova, T.N., Sergeeva, L.I. et al. Morphogenesis of potato plants in vitro. I. Effect of light quality and hormones. J Plant Growth Regul 13, 143–146 (1994). https://doi.org/10.1007/BF00196378

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  • DOI: https://doi.org/10.1007/BF00196378

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