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Continuous separation of proteins by poly(vinyl alcohol) shielded Multichannel Flow Electrophoresis

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Biotechnology Techniques

Summary

A hydrophilic polysulphone membrane spaced multicompartment electrolyzer for Multichannel Flow Electrophoresis (MFE) was developed. Application of poly(vinyl alcohol) (PVA) in MFE resulted in a 35% increase of protein transmembrane flux. Continuous separation of bovine serum albumin (BSA) and haemoglobin bovine blood (HBB) model mixture by PVA shielded MFE yielded 56mg BSA and 48mg HBB per hour. The average recovery was 65%.

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References

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Authors and Affiliations

  1. Dept. of Chemical Engineering, Tsinghua University, 100084, Beijing, People's Republic China

    Zheng Liu, Jin Wang, Zheng Huang, Fuxin Ding, Zhongyao Shen & Naiju Yuan

Authors
  1. Zheng Liu
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  2. Jin Wang
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  3. Zheng Huang
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  4. Fuxin Ding
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  5. Zhongyao Shen
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  6. Naiju Yuan
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Liu, Z., Wang, J., Huang, Z. et al. Continuous separation of proteins by poly(vinyl alcohol) shielded Multichannel Flow Electrophoresis. Biotechnol Tech 10, 253–256 (1996). https://doi.org/10.1007/BF00184024

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  • DOI: https://doi.org/10.1007/BF00184024

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