Summary
The α-chymotrypsin-catalyzed transesterification between N-trifluoroacetyl-DL-phenylalanine trifluoroethyl ester and 1-propanol was carried out in a variety of organic solvents. The addition of small quantities of water enhanced both the rate of reaction and enantioselectivity. A high enantioselectivity was achieved in ethyl acetate (E = 120), diethyl ether (86), or acetonitrile (60). The competing hydrolysis became significant at water content higher than 0.5% (w/w).
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Kawashiro, K., Sugahara, H., Sugiyama, S. et al. Effects of water content on the enantioselectivity of α-chymotrypsin catalysis in organic media. Biotechnol Lett 17, 1161–1166 (1995). https://doi.org/10.1007/BF00128378
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DOI: https://doi.org/10.1007/BF00128378