Abstract
We investigated the presence ofBorrelia burgdorferi sensu lato inIxodes ricinus ticks collected in a Lyme borreliosis (LB) endemic region of northern Croatia. Ticks (n=124) were collected at five locations and analysed by the polymerase chain reaction (PCR). A DNA fragment from the internal transcribed spacer (ITS2) ofI. ricinus was detected in all tick lysates, indicating that PCR inhibitors were not present.Borrelia burgdorferi sensu lato DNA was detected in 56 out of 124 ticks (45%). Four genomic groups were identified:Borrelia afzelii (n=26),Borrelia garinii (n=5), group VS116 (n=5) andB. burgdorferi sensu stricto (n=1). Mixed infections ofB. afzelii with group VS116 (n=10) andB. afzelii withB. burgdorferi sensu stricto (n=1) were also detected. Eight ticks containedB. burgdorferi sensu lato, which could not be typed. The detection ofB. afzelii andB. garinii in ticks was in agreement with manifestations of LB found locally. The occurrence of group VS116 in northern Croatia and in an earlier study in The Netherlands, infers that this genomic group may be well established in EuropeanI. ricinus.
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Assous, M.V., Postic, D., Paul, G., Nevot, P. and Baranton, G. 1993. Western blot analysis of sera from Lyme borreliosis patients according to the genomic species of theBorrelia strains used as antigens. Eur. J. Clin. Microbiol. Infect. Dis. 12: 261–268.
Baranton, G., Postic, D., Saint Girons, I., Boerlin, P., Piffaretti, J.-C., Assous, M. and Grimont, P.A.D. 1992. Delineation ofBorrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int. J. System. Bacteriol. 42: 378–383.
Barbour, A.G., Burgdorfer, W., Hayes, S.F., Péter, O. and Aeschlimann, A. 1983. Isolation of a cultivable spirochete fromIxodes ricinus ticks of Switzerland. Curr. Microbiol. 8: 123–126.
Burek, V., Mišić-Mayerus, L. and Maretić, T. 1992. Antibodies toBorrelia burgdorferi in various population groups in Croatia. Scand. J. Infect. Dis. 24: 683–684.
Burgdorfer, W., Barbour, A.G., Hayes, S.F., Benach, J.L., Grunwaldt, E. and Davies, J.P. 1982. Lyme disease—a tick-borne spirochetosis? Science 216: 1317–1319.
Canica, M.M., Nato, F., du Merle, L., Mazie, J.C., Baranton, G. and Postic, D. 1993. Monoclonal antibodies for the identification ofBorrelia afzelii sp. nov. associated with late cutaneous manifestations of Lyme borreliosis. Scand. J. Infect. Dis. 25: 441–448.
Demaerschalck, I., Ben Massoud, A., De Kesel, M., Hoyois, B., Lobet, Y., Hoet, P., Bigaignon, G., Bollen, A. and Godfroid, E. 1995. Simultaneous presence of differentBorrelia burgdorferi sensu lato genospecies in biological fluids of Lyme disease patients. J. Clin. Microbiol. 33: 602–608.
Eiffert, H., Ohlenbusch, A., Christen, H.-J., Thomssen, R., Spielman, A. and Matuschka, F.-R. 1995. Nondifferentiation between Lyme disease spirochetes from vector ticks and human cerebrospinal fluid. J. Infect. Dis. 171: 476–479.
Fahrer, H., Van der Linden, S.M., Sauvain, M.J., Gern, L., Zhioua, E. and Aeschlimann, A. 1991. The prevalence and incidence of clinical and asymptomatic Lyme borreiosis in a population at risk. J. Infect. Dis. 163: 305–310.
Golubić, D. and Tkalec-Makovec, N. 1992. Clinical, epidemiological and serological characteristic of Lyme borreliosis in Medjimurje. Acta Med. 18: 103–110.
Guy, E.C. and Stanek, G. 1991. Detection ofBorrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction. J. Clin. Pathol. 44: 610–611.
Halouzka, J., Juřicová, Z., Mátlová, L. and Hubalek, Z. 1995.Borreliae in larvalIxodes ricinus ticks. Med. Vet. Entomol. 9: 205–206.
Leuba-Garcia, S., Kramer, M.D., Wallich, R. and Gern, L. 1994. Characterization ofBorrelia burgdorferi isolated from different organs ofIxodes ricinus ticks collected in Nature. Zentralbl. Bakt. Hyg. 280: 468–475.
Magnarelli, L.A., Anderson, J.F. and Cartter, M.L. 1993. Geographic distribution of white-tailed deer with ticks and antibodies toBorrelia burgdorferi in Connecticut. Yale J. Biol. Med. 66: 19–26.
Mohar, N. 1982. Erythema chronicum migrans. Clinical and epidemiological observations on Rijeka district. Acta Dermatol. Jug. 9: 155–158.
Nakao, M. and Miyamoto, K. 1995. Mixed infection of differentBorrelia species amongApodemus speciosus mice in Hokkaido, Japan. J. Clin. Microbiol. 33: 490–492.
Nakao, M., Miyamoto, K. and Fukunaga, M. 1994. Lyme disease spirochetes in Japan: enzootic transmission cycles in birds, rodents, andIxodes persulcatus ticks. J. Infect. Dis. 170: 878–882.
Nohlmans, L.M.K.E., De Boer, R., Van den Bogaard, A.E.J.M. and Van Boven, C.P.A. 1995. Genotypic and phenotypic analysis ofBorrelia burgdorferi isolates from The Netherlands. J. Clin. Microbiol. 33: 119–125.
Péter, O. and Bretz, A.G. 1992. Polymorphism of outer surface proteins ofBorrelia burgdorferi as a tool for classification. Zentralbl. Bakt. Hyg. 277: 28–33.
Pichon, B., Godfroid, E., Hoyois, B., Bollen, A., Rodhain, F. and Perez-Eid, C. 1995. Simultaneous infection ofIxodes ricinus nymphs by twoBorrelia burgdorferi sensu lato species: implications for clinical manifestations. Emerging Infect. Dis. 1: 89–90.
Postic, D. and Baranton, G. 1994. Molecular fingerprinting and phylogeny ofBorrelia burgdorferi sensu lato. In Present status of Lyme disease and biology of LymeBorrelia, Y. Yanaghari and T. Masuzawa (eds), pp. 133–147. Proceedings of the International Symposium on Lyme Disease in Japan, Kanzanji, Hamamatsu, Shizuoka, Japan.
Postic, D., Assous, M., Grimont, P.A.D. and Baranton, G. 1994. Diversity ofBorrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism ofrrf(5S)-rrl(23S) intergenic spacer amplicons. Int. J. System. Bacteriol. 44: 743–752.
Rijpkema, S., Nieuwenhuijs, J., Franssen, F.F.J. and Jongejan, F. 1994. Infection rates ofBorrelia burgdorferi in different instars ofIxodes ricinus ticks from the Dutch North Sea Island of Ameland. Exp. Appl. Acarol. 18: 531–542.
Rijpkema, S.G.T., Molkenboer, M.J.C.H., Schouls, L.M., Jongejan, F. and Schellekens, J.F.P. 1995. Simultaneous detection and genotyping of three genomic groups ofBorrelia burgdorferi sensu lato in DutchIxodes ricinus ticks by characterization of the amplified intergenic spacer region between 5S and 23S rRNA genes. J. Clin. Microbiol. 33: 3091–3095.
Steere, A.C. 1989. Lyme disease. N. Engl. J. Med. 321: 586–596.
Tälleklint, L. and Jaenson, T.G.T. 1993. Maintenance by hares of EuropeanBorrelia burgdorferi in ecosystems without rodents. J. Med. Entomol. 30: 273–276.
Van Dam, A.P., Kuiper, H., Vos, K., Widjojokusumo, A., De Jongh, B.M., Spanjaard, L., Ramselaar, A.C.P., Kramer, M.D. and Dankert, J. 1993. Different genospecies ofBorrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis. Clin. Infect. Dis. 17: 708–717.
Webster, P. and Frandsen, F. 1994. Prevalence of antibodies toBorrelia burgdorferi in Danish deer. APMIS 102: 287–290.
Wesson, D.M., Mclain, D.K., Oliver, J.H., Piesman, J. and Collins, F.H. 1993. Investigation of the validity of species status ofIxodes dammini (Acari: Ixodidae) using rDNA. Proc. Natl Acad. Sci. USA 90: 10221–10225.
Wienecke, R., Neubert, U. and Volkenandt, M. 1993. Cross immunity among types ofBorrelia burgdorferi. Lancet 342: 435.
Wilske, B.A., Preac-Mursic, V., Göbel, U.B., Graf, B., Jauris, S., Soutschek, E., Schwab, E. and Zumstein, G. 1993. An OspA serotyping system forBorrelia burgdorferi based on the reactivity with monoclonal antibodies and OspA sequence analysis. J. Clin. Microbiol. 31: 340–350.
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Rijpkema, S., Golubć, D., Molkenboer, M. et al. Identification of four genomic groups ofBorrelia burgdorferi sensu lato inIxodes ricinus ticks collected in a Lyme borreliosis endemic region of northern Croatia. Exp Appl Acarol 20, 23–30 (1996). https://doi.org/10.1007/BF00051474
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DOI: https://doi.org/10.1007/BF00051474