Abstract
Three mitochondrial plasmids (1704, 1695 and 1478 bp) were isolated from sterile cytoplasms of Vicia faba L. and cloned into a bacterial plasmid vector. Their nucleotide sequence was found to be 99 to 100% homologous to their counterparts isolated from a fertile cytoplasm (J.A. Wahleithner and D.R. Wolstenholme, Curr Genet 12 (1987) 55–76). Several overlapping transcripts were localized in the region which is unique to each of the three plasmids. S1 nuclease mapping indicated for all of them several 3′ termini but a unique 5′ boundary which was located downstream of the consensus sequence CNTAAGTGANNNNNGAA also found at the transcript 5′ boundary of other plant mitochondrial plasmids. Southern blot hybridization with nuclear DNA indicated the presence of nuclear sequences homologous to each plasmid.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abbott AG, O'Dell M, Flavell RB: Quantitative variation in components of the maize mitochondrial genome between tissues and between plants with different male-sterile cytoplasms. Plant Mol Biol 4: 233–240 (1985).
Bailey-Serres J, Leroy Ph, Jones SS, Wahleithner JA, Wolstenholme DR: Size distribution of circular molecules in plant mitochondrial DNAs. Curr Genet 12: 49–53 (1987).
Barnes WM, Bevan M, Son PH: Kilo-sequencing: creation of an ordered nest of asymmetric deletions across a large target sequence carried on phage M13. Meth Enzymol 101: 98–122 (1983).
Berk AJ, Sharp PA: Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12: 721–732 (1977).
Boutry M, Briquet M: Mitochondrial modifications associated with the cytoplasmic male sterility in faba beans. Eur J Biochem 127: 129–135 (1982).
Brow GG, Auchincloss AH, Covello PS, Gray MW, Menassa R, Singh M: Characterization of transcription initiation sites on the soybean mitochondrial genome allows identification of a transcription-associated motif. Mol Gen Genet 228: 345–355 (1991).
de la Canal L, Crouzillat D, Flamand MC, Perrault A, Boutry M, Ledoigt G: Nucleotide sequence and transcriptional analysis of a mitochondrial plasmid from a cytoplasmic male sterile line of sunflower. Theor Appl Genet 81: 812–818 (1991).
Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ: Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 24: 5294–5299 (1979).
Covello PS, Gray MW: RNA editing in plant mitochondria. Nature 341: 662–666 (1989).
Crouzillat D, Gentzbittel L, de la Canal L, Vary C, Perrault A, Nicolas P, Ledoigt G: Properties and nucleotide sequence of a mitochondrial plasmid from sunflower. Curr Genet 15: 283–289 (1989).
Dente L, Cesareni G, Cortese R: pEMBL: A new family of single stranded plasmids. Nucl Acids Res 11: 1645–1655 (1983).
Draper J, Scott R, Armitage P, Walden R: Transformation and Gene Expression: A Laboratory Manual. Black-well, Oxford/London/Edinburgh (1988).
Eisner GI, Negruk VI, Cherny DJ, Alexandrov AA, Shemyakin MF, Butenko RG: Circular DNAs in mitochondria of Vicia faba. FEBS Lett 172: 67–69 (1984).
Fukuchi M, Shikanai T, Kossykh VG, Yamada Y: Analysis of nuclear sequences homologous to the B4 plasmid-like DNA of rice mitochondria; evidence for sequence transfer from mitochondria to nuclei. Curr Genet 20: 487–494 (1991).
Glisin V, Crvenjakov R, Byus C: Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13: 2633–2637 (1974).
Goblet JP, Boutry M, Duc G, Briquet M: Mitochondrial plasmid-like molecules in fertile and male sterile Vicia faba L. Plant Mol Biol 2: 305–309 (1983).
Goblet JP, Flamand MC, Briquet M: A mitochondrial plasmid specifically associated with male sterility and its relation with other mitochondrial plasmids in Vicia faba L. Curr Genet 9: 423–426 (1985).
Gualberto JM, Lamattina L, Bonnard G, Weil JH, Gwenberger JM: RNA editing in wheat mitochondria results in the conservation of protein sequences. Nature 341: 660–662 (1989).
Hansen B, Marcker KA: DNA sequence and transcription of a DNA minicircle isolated from male-fertile sugarbeet mitochondria. Nucl Acids Res 12: 4747–4756 (1984).
Hiesel R, Wissinger B, Schuster W, Brenicke A: RNA editing in plant mitochondria. Science 246: 1632–1634 (1989).
Lonsdale DM: The plant mitochondrial genome. In: The Biochemistry of Plants, Vol. 15, pp. 229–295. Academic Press, New York (1989).
Negruk VI, Cherny DI, Nikiforova JD, Aleksandrov AA, Butenko RG: Isolation and characterization of minicircular DNAs found in mitochondrial fraction of Vicia faba. FEBS Lett 142: 115–117 (1982).
Perez Ch, Dujon B, Heizmann Ph, Berville A: Sequence of a mitochondrial plasmid of sunflower (Helianthus annuus) and its relationship to other mitochondrial plasmids. Plant Sci 58: 59–69 (1988).
Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).
Shikanai T, Yang Z-Q, Yamada Y: Nucleotide sequence and molecular characterization of plasmid-like DNAs from mitochondria of cytoplasmic male-sterile rice. Curr Genet 15: 349–354 (1989).
Stern DB, Newton KJ: Isolation of intact plant mitochondrial RNA using aurintricarboxylic acid. Meth Enzymol 118: 488–496 (1983).
Wahleithner JA, Wolstenholme DR: Mitochondrial plasmid DNAs of faba bean: nucleotide sequences, complex secondary structures, and transcription. Curr Genet 12: 55–67 (1987).
Wahleithner JA, Wolstenholme DR: Origin and direction of replication in mitochondrial plasmid DNAs of faba bean, Vicia faba. Curr Genet 14: 163–170 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Flamand, M.C., Goblet, J.P., Duc, G. et al. Sequence and transcription analysis of mitochondrial plasmids isolated from cytoplasmic male-sterile lines of Vicia faba . Plant Mol Biol 19, 913–923 (1992). https://doi.org/10.1007/BF00040524
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00040524